alexa Expansion of T regulatory Cells in Lepromatous Leprosy
ISSN: 2161-0703

Journal of Medical Microbiology & Diagnosis
Open Access

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Research Article

Expansion of T regulatory Cells in Lepromatous Leprosy is Mediated by Phenolic Glycolipid-1

Bhavyata Dua1, Bhawna Sharma1, Joy Kumar Chakma1, Mamta Arora1, Rekha Bhadauria2, Dipendra Kumar Mitra3 and Beenu Joshi1*

1National JALMA Institute for Leprosy and Other Mycobacterial Diseases (ICMR), Agra, India

2School of Studies in Botany, Jiwaji University, Gwalior, India

3All India Institute of Medical Sciences (ICMR), New Delhi, India

Corresponding Author:
Beenu Joshi
Scientist F, Department of Immunology,
National JALMA Institute for Leprosy and
Other Mycobacterial Diseases (ICMR)
Tajganj, Agra-282004, India
Tel no: +91-562-2331756
Fax no: +91-562-2331755
E-mail: [email protected]

Received date: January 11, 2016; Accepted date: April 12, 2016; Published date: April 20, 2016

Citation: Dua B, Sharma B, Chakma JK, Arora M, Bhadauria R, et al. (2016) Expansion of T regulatory cells in lepromatous leprosy is mediated by Phenolic glycolipid-1. J Med Microb Diagn 5:226. doi:10.4172/2161-0703.1000226

Copyright: © 2016 Dua B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

In leprosy, lepromatous form of the disease is more severe and results from suppression of T cell response. T regulatory cells which suppress T cell response has been found in higher frequency in blood and at the site of infection in leprosy. Therefore, present study was carried out to evaluate the role of Mycobacterium leprae antigens whole cell sonicate (WCS) and especially phenolic glycolipid-1 (PGL-1), which is known for its suppressive nature, in the induction of T regulatory cells expansion in peripheral blood of leprosy patients. For this purpose peripheral blood mononuclear cells (PBMCs) of different category of leprosy patients and healthy controls were stimulated with M. leprae antigens in vitro and percentage of T regulatory cells was determined by flow cytometry. We found higher frequency of T regulatory cells in PBMCs of untreated borderline lepromatous/lepromatous leprosy (BL/LL) patients. Further, PBMCs of untreated BL/LL patients also showed higher percentage of T regs after stimulation with PGL-1. Antigen mediated expansion of T regulatory cells was also supported by results of Carboxy fluorescein succinimidyl ester (CFSE) proliferation assay. None of the antigen induced T regs expansion in healthy controls, untreated tuberculoid/borderline tuberculoid (TT/BT) leprosy patients and treated leprosy patients. Therefore it is suggested that increased frequency of T regs in BL/LL patients may be due to the induction of T regs expansion mediated by PGL-1 of M. leprae and this high percentage of T regs resulted in T cell suppression in lepromatous disease.

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