Experimental Study on the Connection of Nigral Apoptosis of Normal Rats and Rats of Parkinson Disease with Different Doses of Levodopa
|Cao Fei1*, Sun Sheng-Gang1, Cao Xue-Bing1, Wang Tao1, Cheng Ji-Xiang1, Mei Yuan-Wu1, Tong E-Tang1 and Luo Fang2|
|1Department of Neurology, Union Hospital, Tong-ji Medical College, Hua-zhong Scientific and Technologic University, Wuhan, PR China|
|2Trade and Financial Hospital of Hubei Province, Wuhan, PR China|
|Corresponding Author :||Cao Fei
Department of Neurology
Tongji Medical College
Huazhong University of Science and Technology
Wuhan, PR China
E-mail: [email protected]
|Received April 11, 2013; Accepted July 28, 2013; Published July 31, 2013|
|Citation: Fei C, Sheng-Gang S, Xue-Bing C, Tao W, Ji-Xiang C, et al. (2013) Experimental Study on the Connection of Nigral Apoptosis of Normal Rats and Rats of Parkinson Disease with Different Doses of Levodopa. J Neurol Disord 1:125. doi: 10.4172/2329-6895.1000125|
|Copyright: © 2013 Fei C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Objective: To evaluate the neuronal toxicical effects induced by levodopa and explore the method in which PD can be treated reasonably with levodopa.
Method: The rat models of Parkinson disease (PD) were made by injecting stereotaxically 6-OHDA to right side of the mesencephic ventral tegmental area (VTA) and substantia nigra pars compacta (SNc). The PD rats were treated intraperitoneally with different dosages (10 mg/kg/d, 50 mg/kg/d, 100 mg/kg/d) and different durations (1 d, 3 d, 5 d, 7 d) of levodopa. At the same time, we observed the injury of substantia nigral by the way of treating PD rats with levodopa for 7 days and then stopped for 7 days. In this study, we used TUNEL to detect the injury of substantia nigra by different doses and durations of levodopa.
Results: The number of substantia nigral apoptosis was increased with the changes of doses and durations of levodopa.
Conclusions: If we use levodopa intermittently or as little as possible, the toxical action of levodopa may be reduced.