Exploration of Steroidoginic Activity of Ketorolac on Isolated Leydig Cells of Goat (Capra hircus) In Vitro: Part II
Al-Bayati MA* and AI-Luhapy ZZA
Pharmacology and Toxicology, Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq
- *Corresponding Author:
- Mohanad A Al-Bayati
Pharmacology and Toxicology, Department of Physiology and Pharmacology
College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq
E-mail: [email protected]
Received Date: June 02, 2016; Accepted Date: November 21, 2016; Published Date: November 25, 2016
Citation: Al-Bayati MA, AI-Luhapy ZZA (2016) Exploration of Steroidoginic Activity of Ketorolac on Isolated Leydig Cells of Goat (Capra hircus) in vitro: Part II. J Vet Sci Technol 7:404. doi: 10.4172/2157-7579.1000404
Copyright: © 2016 Al-Bayati MA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In the present thesis was conducted to clarify the effect of ketorolac (NSAID) on the Leydig cells of goats to achieve the ketorolac event, by the evaluation steroidoginic activity of ketorolac series on Leydig cell concentrations in vitro. Mitochondrial defects reduction of functional processes of sterodoginc activity reduction of testosterone levels via mitotracker probe indicator, ketorolac may be affected on the steroidogenic acute regulatory (STAR) protein and translocator protein (TSPO). Ketorolac might mutilation and impair the cells were via the uncoupling of oxidative phosphorylation in the cells followed by uncouple mitochondrial respiratory chain as well as ATP depletion. Testosterone level displays in all treated maneuvers LH, LH-GnRH and GnRH in ketorolac challenge decrease testosterone levels at a concentration of ketorolac from 0.6-0.15 mM with the exception in GNRH – ketorolac maneuvers result showed an increase in testosterone level in 0.03-0.06 mM, then fall up down other maneuvers of treatment. The testosterone synthesis in Leydig cells of mammalian is achieved almost motivated by the binding of luteinizing hormone (LH) to the plasma membrane of Leydig cell receptors efficaciously, that lead to creation of c.AMP Inhibition of LTB4 that due to direct inhibition of lipoxygenase action, and had a direct effect to reduce LTB4 and may be various eicosanoids also 12 (S) - hydroxyeicosatetraenoic acid and 15 (S) - hydroxyeicosatetraenoic acid binds to and activates LTB.