alexa
Reach Us +44-1647-403003
Exposure to Interferon andgamma; Decreases Levels and Activity of Key Cell Cycle Proteins Resulting in Severe Growth Arrest of the Human Non-Transformed Cell Line, WISH | Abstract
ISSN: 1948-5956

Journal of Cancer Science & Therapy
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Exposure to Interferon γ Decreases Levels and Activity of Key Cell Cycle Proteins Resulting in Severe Growth Arrest of the Human Non-Transformed Cell Line, WISH

Surabhi Vashistha and Parthasarathi Ajitkumar*

Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore - 560012, Karnataka, India

*Corresponding Author:
Dr. Parthasarathi Ajitkumar
Department of Microbiology and Cell Biology
Indian Institute of Science, Bangalore–560012
Karnataka, India
Tel: 91-80-22932344
Fax: 91-80-23602697
E-mail: [email protected]

Received date: September 23,2010; Accepted date: November 02,2010; Published date: November 02,2010

Citation: Vashistha S, Ajitkumar P (2011) Exposure to Interferon γ Decreases Levels and Activity of Key Cell Cycle Proteins Resulting in Severe Growth Arrest of the Human Non-Transformed Cell Line, WISH. J Cancer Sci Ther 3: 013-019. doi: 10.4172/1948-5956.1000050

Copyright: © 2011 Vashistha S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

Abstract

Interferon ? (IFN?), a potent inhibitor of proliferation,inducer of apoptosis and an immune modulator of mammalian cells, has been used as an anticancer agent in cancer therapy. Several molecular mechanisms, depending upon the differences in the lineage of transformed cell targets, have been elucidated for the growth inhibition or apoptosis of target cancer cells by IFN?. However, its mechanism of action on normal cells needs to be understood from the point of view of: (i) The effect of IFN? on non-transformed cell line and (ii) The side effect of interferon therapy on normal cells in cancer patients. Using the non-transformed cell line, human foetal epithelial cell line (WISH), our earlier studies had shown that IFN? detains cells at a point prior to the activation step of cyclin dependent kinase 2 (CDK2) in the G1 phase of cell cycle. In the present study, we identifi ed significant reduction in the levels and/or activity of cyclin E-CDK2, CDC25A phosphatase, cyclin H, cyclin E, cyclin D, p21 and p27. The drastic decrease in the levels and/or activity of cyclin E and/or of cyclin E-CDK2 complex might have caused growth arrest of WISH cells by IFN?.

Keywords

Share This Page
Top