alexa Expression of M-CSF, TNF-β, IFN-γ, and IL-10 in Rats with Liver Cirrhosis and Hypersplenism and its Significance | OMICS International
ISSN: 2167-1095

Journal of Hypertension: Open Access
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Research Article

Expression of M-CSF, TNF-β, IFN-γ, and IL-10 in Rats with Liver Cirrhosis and Hypersplenism and its Significance

Yunfu Lv1*, Jie Deng2, Bookyong Yu2, Qingqing Li1,2 and Xinqiu Li3

1Department of General Surgery, Hainan Provincial People's Hospital, Haikou, China

2Department of General Surgery, The People's Liberation Army 187 Hospital, Haikou, China

3Hubei Province People's Hospital affiliated to School of Pharmacy, Shiyan, China

*Corresponding Author:
Yunfu Lv
Department of General Surgery
Hainan Provincial People's Hospital
Haikou 570311, China
Tel: 8613687598368
E-mail: [email protected]

Received Date: December 20, 2016; Accepted Date: December 26, 2016; Published Date: December 31, 2016

Citation: Lv Y, Deng J, Yu B, Li Q, Li X (2016) Expression of M-CSF, TNF-β, IFN-γ, and IL-10 in Rats with Liver Cirrhosis and Hypersplenism and its Significance. J Hypertens 5: 234. doi:10.4172/2167-1095.1000234

Copyright: © 2016 Yunfu L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Objective: This study aimed to examine the expression of M-CSF, TNF-β, IFN-γ, and IL-10 in rats with liver cirrhosis and hypersplenism, and to investigate its significance. Methods: Seventy-two healthy male SD rats were randomly divided into a model group (n=60) and a control group (n=12). The rats in the model group were first gavaged with a 40% CCL4/peanut oil solution to develop cirrhosis and hypersplenism. The expression rates and intensities of the pro-inflammatory cytokines M-CSF, TNF-β, and IFN-γ, and the anti-inflammatory cytokine IL-10 in the spleen were measured and compared with the control group. The pro-inflammatory/anti-inflammatory cytokine ratio and its role were analyzed. Results: The positive expression rates of M-CSF, TNF-β, IFN-γ, and IL-10 in the model group (cirrhosis and hypersplenism) were 61.76%, 79.41%, 64.70%, and 88.24%, respectively, which were significantly different (P<0.05) from the 25%, 33.33%, 16.67%, and 50% in the control group. The relative protein expression intensities of M-CSF, TNF-β, IFN-γ, and IL-10 in the model group were 0.63 ± 0.58, 1.06 ± 0.49, 0.99 ± 0.38, and 1.12 ± 0.42, respectively, and were significantly different (P<0.05) from the 0.18 ± 0.12, 0.52 ± 0.27, 0.38 ± 0.28, and 0.60 ± 0.32 in the control group. The relative mRNA expression levels of M-CSF, TNF-β, IFN-γ, and IL-10 in the model group were 2.06 ± 0.11, 4.07 ± 0.19, 2.98 ± 0.11, and 7.94 ± 0.27, respectively, and were significantly different (P<0.05) from the 1.01 ± 0.05, 1.06 ± 0.11, 1.00 ± 0.31, and 1.02 ± 0.08 in the control group. Conclusion: The abnormally increased expression of M-CSF, TNF-β, IFN-γ, and IL-10 in rats with liver cirrhosis and hypersplenism, as well as the abnormal pro-inflammatory/anti-inflammatory cytokine ratio and regulation may play an important role in enhancing the phagocytosis of macrophages and causing peripheral cytopenias.

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