alexa Extended-Spectrum β-Lactamase Enzymes (ESBLs) Pr
ISSN: 2472-1212

Journal of Antimicrobial Agents
Open Access

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Research Article

Extended-Spectrum β-Lactamase Enzymes (ESBLs) Produced by Escherichia coli Urinary Pathogens at Riyadh, Saudi Arabia

Al-Mijalli SHS*

Biology Department, Scientific Section, Princess Norah Bent AbdulRahman University, Saudi Arabia

*Corresponding Author:
Samiah HS Al-Mijalli
Biology Department, Scientific Section,
Princess Norah Bent AbdulRahman University
Riyadh, Saudi Arabia
Tel: +966118220000
E-mail: [email protected]

Received Date: August 04, 2016; Accepted Date: August 31, 2016; Published Date: September 05, 2016

Citation: Al-Mijalli SHS (2016) Extended-Spectrum β-Lactamase Enzymes (ESBLs) Produced by Escherichia coli Urinary Pathogens at Riyadh, Saudi Arabia. J Antimicrob Agents 2:125. doi:10.4172/2472-1212.1000125

Copyright: © 2016 Al-Mijalli SHS. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Background: This study aim was to determine the probable type of β-lactamase gene which is responsible for resistance. It was found that OXA (701 bp) was the main type of β-lactamase (35.7%), CTX-M (569 bp) was second (28.9%), TEM (403 bp) was third (20.5%) and SHV (293 bp) (14.9%) was fourth. The aim and objectives of this study were to investigate the prevalence of ESBLs producing in these bacteria isolated from uropathogenic out-patients and to look for the presence of TEM or SHV, CTX and OXA genes in E. coli. Results: The present study was carried out from the Central Laboratory of Riyadh Hospital in Saudi Arabia from January 2014 to June 2015. Total 116 urine samples were tested bacteriologically and for antibiotic susceptibility using standard procedures, Detection of extended-spectrum β-lactamases and determination of the genotype of β- lactamase of 75 E. coli isolates by PCR: It was found that OXA (701 bp) was the main type of β-lactamase (35.7%), CTX-M (569 bp) was second (28.9%), TEM (403 bp) was third (20.5%) and SHV (293 bp) (14.9%) was fourth. Conclusions: This study showed that the ESBL producing isolates detected PCR with oligonucleotide primers of TEM, SHV, and CTX-M and OXA genes and were carried out on E. coli DNA of 75 isolates. PCR, incorporating the primers for commonly prevalent ESBLs may be a valuable clinical and research tool for characterization of ESBLs

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