Extraction of Anticancerous Enzymes from E.coli and a New Method to Study its ActivityNeha Hanif*
Microbiologist at Dr. Essa Laboratory and Diagnostic Centre, Jinnah University for Women Karachi, Pakistan
- *Corresponding Author:
- Neha Hanif
Microbiologist at Dr. Essa Laboratory and Diagnostic Centre
Jinnah University for Women Karachi, Pakistan
E-mail: nehahani[email protected]
Received Date: February 11, 2017; Accepted Date: February 28, 2017; Published Date: March 06, 2017
Citation: Hanif N (2017) Extraction of Anticancerous Enzymes from E.coli and a New Method to Study its Activity. J Bacteriol Parasitol 8:301.doi: 10.4172/2155-9597.1000301
Copyright: © 2017 Hanif N. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use,distribution, and reproduction in any medium, provided the original author and source are credited.
L-asparaginase is the most promising anti-tumour enzyme that reduces the level of L-asparagine (an important nutrient for cancer cells) resulting in cancer cell starvation which leads to the cell death. There are many sources of asparaginase but bacterial source i.e. E.coli and Erwinia spp is mostly use as a therapeutic agent against leukaemia. In this study screening of different bacteria was done by rapid plate assay for asparaginase producing capability then intracellular and extracellular Asparaginase were extracted from its potent producer i.e. E.coli which was isolated from sewage water. The activity of enzyme was determined by using new procedure i.e. rapid activity analysis on agar plate and in tubes. Our results shows that an intracellular asparaginase enzyme was found to be more active against asparagines as compare to the extracellular enzyme. This enzyme isn’t only the requirement of therapy for tumour cells but also it is use in food industry so in upcoming years the demand for asparaginase will get increase and our new method for intracellular enzyme extraction and activity analysis method (quantitative and qualitative method) will gain its importance for being easy, less expensive, using less volume of enzymatic extracts and giving better results as compare to the any other activity analysis method.