Extraction of Cefquinome from Food by Magnetic Molecularly Imprinted PolymerAggarwal S1, Rajput YS1*, Sharma R2 and Pandey AK1
- *Corresponding Author:
- Rajput YS
Emeritus Scientist, Animal Biochemistry Division
National Dairy Research Institute
Email: [email protected]
Received date: October 26, 2016; Accepted date: December 02, 2016; Published date: December 05, 2016
Citation: Aggarwal S, Rajput YS, Sharma R, Pandey AK (2016) Extraction of Cefquinome from Food by Magnetic Molecularly Imprinted Polymer. Pharm Anal Acta 7:527. doi: 10.4172/2153-2435.1000527
Copyright: © 2016 Aggarwal S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objectives: Cefquinome is a fourth generation cephalosporin with broad spectrum antimicrobial activity. It is commonly used against respiratory tract diseases, acute mastitis, calf septicemia and foot rot in livestock. Clean-up step in sample preparation is invariably required for avoiding interferences in assay from other components. It is envisaged to prepare molecular imprinted based polymer with desired selectivity for cefquinome. Method: Imprinted polymer was synthesized over the surface of magnetite using methacrylate (monomer) acid and ethyleneglycoldimethacrylate (crosslinker) in the presence of cefquinome (imprint molecule). Magnetite was prepared by co-precipitation of 1.98 g FeCl2.4H2O and 5.41 g FeCl3.6H2O. Magnetic non-imprinted polymer was prepared similarly in absence of cefquinome. Selectivity in different solvent was calculated from partition coefficient values in imprinted and non-imprinted polymers. Result: Selectivity of imprinted polymer over non-imprinted polymer was dependent on nature of solvent and pH. Binding of cefquinome was highly selective in acetonitrile. Imprinted polymer lacks selectivity in methanol and 1M NaCl. Ionic interaction between quinolinium ion in cefquinome and carboxylate anion in polymer appears to be major force for recognition of cefquinome by imprinted polymer. Prepared polymer extracted cefquinome from milk, honey, egg and water and extraction efficiency was in the range of 74-93.7%. Conclusion: Selectivity of magnetic imprinted polymer must be determined in different solvents and this will enable selecting binding and elution conditions during extraction of imprint molecule.