Feasibility Studies on Vitrification of Persian Sturgeon (Acipenser persicus)Embryos
Saeide Keivanloo* and Mohammad Sudagar
Department of Fisheries, University of Agricultural and Natural Resources, Gorgan, 49138-15749, Iran
- *Corresponding Author:
- Keivanloo S
Department of Fisheries
University of Agricultural and Natural Resources
E-mail: [email protected]
Received Date: November 28, 2012; Accepted Date: January 16, 2013; Published Date: January 28, 2013
Citation: Keivanloo S, Sudagar M (2013) Feasibility Studies on Vitrification of Persian Sturgeon (Acipenser persicus) Embryos. J Aquac Res Development 4:172. doi:10.4172/2155-9546.1000172
Copyright: © 2013 Keivanloo S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The feasibility of cryopreservation by vitrification of Persian sturgeon (Acipenser persicus) embryos at 48 h post-fertilization stage was investigated. Vitrification is considered the most promising option. Many factors are involved in the success of the process. The choice of a proper vitrificant solutions and temperature for thawing, were the parameters considered in the present study. Six vitrificant solutions (V1-V6) were tested using a stepwise incorporation protocol. The tested solutions contained acetamide as the main cryoprotectant +3 other permeable cryoprotectants +3 non-permeable cryoprotectants. Before loading the embryos into tubes, toxicity tested was affected with these solutions. The hatching rate of embryos that had been exposed to the vitrificant solutions was analyzed and the highest hatching rate was obtained with exposure to V1. After thawing (water bath, 0 or 20°C), embryos were incubated until hatched. The highest survival rate (69.69%) was observed in samples frozen with V1 and thawed at 20°C. These results establish that cryopreservation of Persian sturgeons embryos by vitrification is possible.