alexa Fermentation Monitoring of a Co-Culture Process with Saccharomyces cerevisiae and Scheffersomyces stipitis Using Shotgun Proteomics | OMICS International
ISSN: 2155-9821

Journal of Bioprocessing & Biotechniques
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Fermentation Monitoring of a Co-Culture Process with Saccharomyces cerevisiae and Scheffersomyces stipitis Using Shotgun Proteomics

Eric L Huang and Mark G Lefsrud*
Bioresource Engineering, McGill University, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, Quebec, H9X 3V9, Canada
Corresponding Author : Mark G Lefsrud
Bioresource Engineering, McGill University
21111 Lakeshore Road, Ste-Anne-de-Bellevue
Quebec, H9X 3V9, Canada
Tel: 514-398-7967
Fax: 514-398-8387
Email: [email protected]
Received October 27, 2013; Accepted January 02, 2014; Published January 09, 2014
Citation: Huang EL, Lefsrud MG (2014) Fermentation Monitoring of a Co- Culture Process with Saccharomyces cerevisiae and Scheffersomyces stipitis Using Shotgun Proteomics. J Bioprocess Biotech 4:144.doi:10.4172/2155-9821.1000144
Copyright: © 2014 Huang EL, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and and source are credited.

Abstract

Co-culture processes present the opportunity to produce value-added products from economical raw materials, but there lacks a high-throughput fermentation monitoring technique to study the temporal physiology of fermentation organisms in co-culture processes. In this study, we applied shotgun proteomics to investigate a co-culture process of Saccharomyces cerevisiae and Scheffersomyces stipitis, and we monitored the fermentation until glucose depletion. Three time points were taken for proteomics analysis at 11.5 hour, 18.5 hour and 32 hour, representing transition into diauxic shift. Using label-free quantitation, we observed cellular dynamic within 20-hour time frame. We distinguished the proteome between two yeasts, and the most abundant proteins of S. stipitis and S. cerevisiae contained expected processes of glycolytic enzymes, histones, heat shock proteins, ribosomal proteins and F1F0-ATPase. After glucose depletion, we identified up-regulations of S. stipitis malate synthase and isocitrate lyase as key enzymes in glyoxylate cycle and gluconeogenesis. Increased expression of S. stipitis histone 2B was observed in diauxic shift, and histone acetylation was suggested by up-regulation of acetyl-CoA synthetase. Without the presence of xylose, we observed induction of NAD(P)H-dependent D-xylose reductase (Xyl1p) as early as 11.5-hour before glucose depletion. We also observed the expression of D-xylulose reductase after glucose depletion without xylose induction. Further study is needed to investigate the cause of derepression signals for xylose oxo-reductive pathway. Without cellulose induction, the up-regulation of S. stipitis endo-1,4-beta-glucanase suggested S. stipites’ strategy in diversifying carbon choices after glucose repression. This research demonstrated the application of shotgun proteomics in high-throughput monitoring of complex co-culture system and able to elucidate the temporal physiology of S. stipitis.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version