Fiber Optic Biosensor for the Detection of Cd in Milk
Neelam Verma*, Sachin Kumar and Hardeep Kaur
Department of Biotechnology, Punjabi University, Patiala, India
- *Corresponding Author:
- Dr. Neelam Verma
Department of Biotechnology
Fax: +91-175-2286412, 2283073
E-mail: [email protected]
Received Date: October 05, 2010; Accepted Date: October 19, 2010; Published Date: October 20, 2010
Citation: Verma N, Kumar S, Kaur H (2010) Fiber Optic Biosensor for the Detection of Cd in Milk. J Biosens Bioelectron 1:102. doi: 10.4172/2155-6210.1000102
Copyright: © 2010 Verma N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A novel absorption-transmission based, miniaturized fi ber optic biosensor has been developed for the detection of cadmium in milk. Biosensor constitutes Bacillus badius whole cells with phenol red as an indicator co-immobilized onto circular plastic discs with sol-gel approach and a fi ber optic transducer system. Inhibition of urease enzyme by cadmium ion has been used as bioassay principle in the study. The detection limit of 0.1 g/l has been achieved. Sample volume could be miniaturized to 10 l; miniaturization of sample volume to this level has never been cited in literature. Storage stability of biocomponent was found to be more than 90 days when stored at 4°C in 10% glycerol. Conclusively the study resulted in development of a quick, reliable, miniaturized biosensor with lower detection limit and longer storage stability.