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Fiber Optic Biosensor for the Detection of Cd in Milk | OMICS International | Abstract
ISSN: 2155-6210

Journal of Biosensors & Bioelectronics
Open Access

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Research Article

Fiber Optic Biosensor for the Detection of Cd in Milk

Neelam Verma*, Sachin Kumar and Hardeep Kaur

Department of Biotechnology, Punjabi University, Patiala, India

*Corresponding Author:
Dr. Neelam Verma
Department of Biotechnology
Punjabi University
Patiala, India
Tel: +91-175-3046263
Fax: +91-175-2286412, 2283073
E-mail: [email protected]

Received Date: October 05, 2010; Accepted Date: October 19, 2010; Published Date: October 20, 2010

Citation: Verma N, Kumar S, Kaur H (2010) Fiber Optic Biosensor for the Detection of Cd in Milk. J Biosens Bioelectron 1:102. doi: 10.4172/2155-6210.1000102

Copyright: © 2010 Verma N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A novel absorption-transmission based, miniaturized fi ber optic biosensor has been developed for the detection of cadmium in milk. Biosensor constitutes Bacillus badius whole cells with phenol red as an indicator co-immobilized onto circular plastic discs with sol-gel approach and a fi ber optic transducer system. Inhibition of urease enzyme by cadmium ion has been used as bioassay principle in the study. The detection limit of 0.1 g/l has been achieved. Sample volume could be miniaturized to 10 l; miniaturization of sample volume to this level has never been cited in literature. Storage stability of biocomponent was found to be more than 90 days when stored at 4°C in 10% glycerol. Conclusively the study resulted in development of a quick, reliable, miniaturized biosensor with lower detection limit and longer storage stability.

Keywords

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