alexa Filamentous Phages Displaying Multivalent Peptide Motives With Specific Affinity To Anodic Alumina Surfaces | OMICS International | Abstract
ISSN: 2155-6210

Journal of Biosensors & Bioelectronics
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Filamentous Phages Displaying Multivalent Peptide Motives With Specific Affinity To Anodic Alumina Surfaces

Joris Proost1*, Geneviève Deschuyteneer2, Ronny Santoro1, Quentin Van Overmeere1, Patrice Soumillion2 and Denis Flandre3

1Division of Materials and Process Engineering, Place Sainte-Barbe 2, Université catholique de Louvain, B-1348 Louvain-la-Neuve, Belgium

2Laboratoire d’Ingénierie des Protéines et des Peptides, Place Croix du Sud 4-5, Université catholique de Louvain, B-1348 Louvain-la-Neuve, Belgium

3Information and Communication Technologies, Electronics and Applied Mathematics, Université catholique de Louvain, B-1348 Louvain-la-Neuve, Belgium

*Corresponding Author:
Joris Proost
Division of Materials and Process Engineering
Place Sainte-Barbe 2
Université catholique de Louvain
B-1348 Louvain-la-Neuve, Belgium
Tel: +32 10 47 93 42
Fax: +32 10 47 40 28
E-mail: [email protected]

Received Date: September 05, 2014; Accepted Date: December 10, 2014; Published Date: March 10, 2015

Citation: Proost J, Deschuyteneer G, Santoro R, Overmeere QV, Soumillion P, et al. (2015) Filamentous Phages Displaying Multivalent Peptide Motives with Specific Affinity to Anodic Alumina Surfaces. J Bioeng Biomed Sci 6:162. doi: 10.4172/2155-6210.1000162

Copyright: © 2015 Proost J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

In this work, we report for the first time on the successful selection and identification of peptide motives that exhibit a specific affinity to anodic alumina surfaces when multivalently displayed on a filamentous phage. It was also demonstrated that, for a selected phage clone, a chemical functionalisation (biotinylation) of the bacteriophage does not deteriorate its specific affinity to anodic alumina. Moreover, such biotinylated bacteriophages, after being immobilised onto an anodic alumina surface, have been shown to allow for the quantitative detection of streptavidine using an ELISA protocol. These results are believed to pave the way for shifting the surface design of integrated biosensing devices from traditional, chemically modified synthetic surfaces, like silane-based self-assembled monolayers, towards molecular linkers based on genetically engineered polypeptides.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

neuropsycho[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7