Veterinary Science & Technology

First Genotype Characterization of Giardia intestinalis Assemblage E from Goat Kids in Bangladesh

Abstract

Mohammad Alamgir Hossain, Sohana Akter Mina, Lolo Wal Marzan, Mohammad Asif Imran Khan Emon, Rasel Das, AMAM Zonaed Siddiki

Giardiasis is an important protozoan disease caused by Giardia and manifested as life-threatening diarrhea in animals and humans worldwide. No study has performed to see zoonotic epidemiology and diversity of Giardia in animals in Bangladesh. Hence, there is a room to characterize Giardia protozoan parasite in animals to find out their roles as a source of human infection. To address this hurdle, fecal samples of 100 goat kids were genotyped by nested PCR amplification of β-giardin gene fragment followed by sequencing and analysis. The total prevalence of Giardia in goat kids was 3% (3/100) and the infection is more widespread in younger, Black Bengal breed and female goat kids. Further analysis of β-giardin gene locus has shown and clustered the Giardia in Assemblage E rather than Assemblages A and B. It suggests the low zoonotic transmission frequency from the goat kids and has least epidemiological significance to humans. Further study on this field is prerequisite in terms of broad geographical areas, age groups, sex and evaluates zoonotic significance along with genetic diversity in other host species as well.