alexa Flexible Electrospun Cellulose Fibers as an Affinity Packing Material for the Separation of Bovine Serum Albumin
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

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Research Article

Flexible Electrospun Cellulose Fibers as an Affinity Packing Material for the Separation of Bovine Serum Albumin

Minoru Miyauchi1, Jianjun Miao2, Trevor J. Simmons1, Jonathan S. Dordick1,2,3,4 and Robert J. Linhardt1,2,3,4*

1Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies and Rensselaer Center for Nanotechnology; Rensselaer Polytechnic Institute, Troy, NY, 12180, U.S.A

2Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies and Rensselaer Center for Nanotechnology; Rensselaer Polytechnic Institute, Troy, NY, 12180, U.S.A

3Department of Biology, Center for Biotechnology and Interdisciplinary Studies and Rensselaer Center for Nanotechnology; Rensselaer Polytechnic Institute, Troy, NY, 12180, U.S.A

4Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies and Rensselaer Center for Nanotechnology; Rensselaer Polytechnic Institute, Troy, NY, 12180, U.S.A

*Corresponding Author:
Robert J. Linhardt
Department of Biomedical Engineering
Center for Biotechnology and Interdisciplinary Studies and
Rensselaer Center for Nanotechnology; RPI
Biotech 4005, Troy, NY, 12180, USA
Tel:
518-276-3405
Fax: -3405
E-mail: [email protected]

Received date: May 07, 2011; Accepted date: August 05, 2011; Published date: August 06, 2011

Citation: Miyauchi M, Miao J, Simmons TJ, Dordick JS, Linhardt RJ (2011) Flexible Electrospun Cellulose Fibers as an Affinity Packing Material for the Separation of Bovine Serum Albumin. J Chromatograph Separat Techniq 2:110. doi:10.4172/2157-7064.1000110

Copyright: © 2011 Miyauchi M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Flexible, well-dispersed and continuous 100-nm diameter cellulose fibers were prepared from an ionic liquid solvent by a novel dry-jet wet-electro spinning process. The ribbon fibers formed were chemically activated and an affinity dye, cibacron blue (CB) was immobilized at 0.22 g CB/g dry fibers loading to the surface of these fibers. The resulting affinity matrix was packed into a chromatography column and the adsorption, desorption and specificity of this matrix for bovine serum albumin (BSA) was studied. These electrospun fibers had a BSA binding capacity of 230 mg/g, nearly twice that of CB-immobilized 100-?m beads and over ten-fold higher capacity that CB-immobilized cellulose fibers prepared by a conventional electrospinning process. The results of this work suggest that chromatography supports of flexible, well-dispersed, continuous nanofibers may offer advantages over conventional supports in affinity separations.

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