alexa Flow Cytometry Analysis of Pro- and Anti-inflammatory Cytokines in Alzheimer's Disease Model Treated with Ginkgo biloba
ISSN: 2155-6210

Journal of Biosensors & Bioelectronics
Open Access

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Research Article

Flow Cytometry Analysis of Pro- and Anti-inflammatory Cytokines in Alzheimer's Disease Model Treated with Ginkgo biloba

Salgado ASI1, Eltchechem CL2, Kerppers II2*, Silva Pereira MC2, Suckow PT2, Gomes JC2, Hosni AP2, Miri AL2 and Carraro E3

1Manual and Postural Therapy School, Londrina State University, Londrina, Brazil

2Neuroanatomy and Neurophysiology Laboratory, Midwest State University, Guarapuava, Brazil

3Immunology Laboratory, Midwest State University, Guarapuava, Brazil

*Corresponding Author:
Ivo Ilvan Kerppers
Neuroanatomy and Neurophysiology Laboratory
Midwest State University, P.O.: 85040-080
Guarapuava, Brazil
Tel: +55 042-3621-1090
E-mail: [email protected]

Received Date: March 28, 2017; Accepted Date: April 12, 2017; Published Date: April 22, 2017

Citation: Salgado ASI, Eltchechem CL, Kerppers II, Silva Pereira MC, Suckow PT, et al. (2017) Flow Cytometry Analysis of Pro- and Anti-inflammatory Cytokines in Alzheimer's Disease Model Treated with Ginkgo biloba. J Biosens Bioelectron 8: 241. doi: 10.4172/2155-6210.1000241

Copyright: © 2017 Salgado ASI, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Alzheimer's disease (AD) is a neurodegenerative disorder that accounts for two-thirds of dementia cases in the elderly and may show preliminary symptoms for decades. The dry leaf extract of Ginkgo biloba (EGb 761) has been used as a dietary supplement for the treatment of mental disorders in both humans and animals. Objective: This study examined the immunological effect of EGb 761 in the treatment of rats with experimental Alzheimer's disease. Methodology: The sample consisted of 40 Wistar male rats, weighing 200-250 grams and divided into 4 groups: 21 days control, 42 days control, 21 days treated, and 42 days treated. Stereotaxic surgery was performed with the coordinates of AP=-3.12, ML=3.6 mm, and DV=3.0; animals were undisturbed for 5 days after surgery and brought back to stereotaxic to be injected with 2 μL (1 mg) of Beta-amiloide25-35 toxin in the CA1 region. Animals in the treated group received 100 mg/kg of EGb 761 by gavage. Animals were euthanized at 21 and 42 days; 1 ml of blood was collected for the flow cytometry analysis of pro- and anti-inflammatory cytokines. Results: A significant difference was observed between the pro-inflammatory cytokines: IL-6 and IFN-γ showed low levels of concentration compared to IL-2 and TNF-α. The IL-4 and IL-10 anti-inflammatory cytokines were present at high levels of concentration in the 21 and 42 days treated groups. Conclusion: EGb 761 was effective in reducing serum levels of pro-inflammatory cytokines, thereby promoting a reduction in the inflammatory process generated by AD.


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