alexa Flow Cytometry as a Tool to Study the Effects of Bacteriocins on Prokaryotic and Eukaryotic Cells
ISSN-2155-9929

Journal of Molecular Biomarkers & Diagnosis
Open Access

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Review Article

Flow Cytometry as a Tool to Study the Effects of Bacteriocins on Prokaryotic and Eukaryotic Cells

Harsh Mathur1,2*, Mary C Rea2,3, Vincenzo Fallico2, Paul D Cotter2,3, Colin Hill1,3 and Paul Ross R3,4

1School of Microbiology, University College Cork, Cork, Ireland

2Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland

3APC Microbiome Institute, University College Cork, Cork, Ireland

4College of Science, Engineering and Food Science, University College Cork, Cork, Ireland

*Corresponding Author:
Harsh Mathur
School of Microbiology, University College Cork
Cork, Ireland and Moorepark Food Research Centre
Teagasc, Fermoy, Cork, Ireland
Tel: +353 (0)21 4902745
E-mail: [email protected] ie, [email protected]

Received date: December 09, 2015;4 Accepted date: January 23, 2016; Published date: January 25, 2016

Citation: Mathur H, Rea MC, Fallico V, Cotter PD, Hill C et al. (2016) Flow Cytometry as a Tool to Study the Effects of Bacteriocins on Prokaryotic and Eukaryotic Cells. J Mol Biomarkers Diagn S8:013. doi:10.4172/2155-9929.S8-013

Copyright: © 2016 Mathur H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

 

Abstract

Researchers use a combination of techniques to study and contrast the impact of antimicrobials, such as bacteriocins, on sensitive and resistant variants. Flow cytometry is one such technique, which allows researchers to evaluate the activity of antimicrobials at a single-cell level in real-time. The generation of an increasing number of probes/dyes that can be used in flow cytometry studies has vastly expanded the potential applications of this technique. Furthermore, flow cytometry has the potential to replace, or at the very least be used as an adjunct to traditional growth-based techniques, including viable plate counts, growth curves, microscopic analysis and cell culture, many of which have limitations when used on their own. Here we review studies conducted using flow cytometry as a technique to assess the impact of antimicrobials from the bacteriocin family on individual cells, either prokaryotic or eukaryotic.

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