Flow-Injection Chemiluminescence Determination of Fleroxacin in Pharmaceutical Preparations and Human UrineHanwen Sun1* and Liqing Li2
- *Corresponding Author:
- Hanwen Sun
College of Chemistry and Environmental Science
Key Laboratory of Analytical Science and Technology of Hebei Province
Baoding, 071002, China
E-mail: [email protected]
Received date: October 24, 2010; Accepted date: December 05, 2010; Published date: December 07, 2010
Citation: Sun H, Li L (2010) Flow-Injection Chemiluminescence Determination of Fleroxacin in Pharmaceutical Preparations and Human Urine. J Chromatograph Separat Techniq 1:104. doi:10.4172/2157-7064.1000104
Copyright: © 2010 Sun H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Quality control of drug fleroxacin dosage, its monitoring in biological fluids, and research of drug’s metabolism and action are an important analytical task. A new chemiluminescence (CL) reaction system was established for the determination of fleroxacin (FLX). The trivalence dysprosium-sensitized CL emission mechanism was investigated by comparing the fluorescence emission with CL spectra. The CL spectra of FLX-KMnO 4 -Na 2 S 2 O 3 -H 6 P 4 O 13 system are from the narrow characteristic emission of Dy 3+ at 482 and 578 nm ( 4 F 9 → 6 H 15/2 , 4 F 9 → 6 H 13/2 ) through the energy transfer from the excited SO 2 * to analyte, followed by intramolecular energy transfer from analyte* to Dy 3+ . The optimum conditions for CL emission were investigated and optimized. The relationships between the relative CL intensity and the concentration of the studied analyte have good linearity. The detection limit for FLX was 3.0×10 -10 g/mL. The relative standard deviation is 2.0% for 11 determinations of FLX at 2.0×10 -6 g/mL. The proposed CL system has been successfully applied for the determination of FLX in the injections and urine sample with satisfactory result. .