Follicular Fluid Activin A and Leptin are not Correlated with IVF Outcome
Mohamed M Shaaban1, Waleed S Ahmed1*, Iman Keshk1 and Hossam El Bahaie2
1Department of Obstetrics and Gynecology, Suez Canal University, Egypt
2Department of clinical pathology, Suez Canal University, Egypt
- *Corresponding Author:
- Waleed S Ahmed
Department of Obstetrics and Gynecology
Suez Canal University
E-mail: [email protected]
Received date: September 24, 2012; Accepted date: November 26, 2012; Published date: November 30, 2012
Citation: Shaaban MM, Ahmed WS, Keshk I, Bahaie HE (2012) Follicular Fluid Activin A and Leptin are not Correlated with IVF Outcome Measures. J Steroids Horm Sci 4:111. doi:10.4172/2157-7536.1000111
Copyright: © 2012 Shaaban MM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objective: The present study is designed to prospectively evaluate relationship between follicular fluid (FF) Leptin and Activin A and IVF outcome measures.
Design: Prospective observational study.
Population: FF Leptin and Activin A were measured in 90 patients undergoing ICSI cycles excluding polycystic ovarian syndrome (PCOS) patients. Follicular fluid samples collected at oocyte retrieval from follicles ≥ 18 mm in diameter were analyzed for Leptin and Activin A using enzyme-linked immunosorbent assay (ELISA) and results were correlated to ICSI outcome.
Main outcome measures: FF leptin and activin A levels, fertilization and pregnancy rates.
Results: For the whole population (90 patients), mean follicular fluid Leptin level was 45.2 ± 26.7 ng/ml and mean body mass index was 25.3 ± 3.3 kg/m². FF Leptin had a positive correlation with BMI (p<0.005). The mean FF Activin A was 880.8 ± 354.6 pg/ml 29/90 patients (32.2%) achieved pregnancy, twenty five of them (86.2%) had more than 50% grade A embryos on day 2 in contrast to 33 patients (54.1%) in the non-pregnant group (P<0.02), this means that pregnancy was positively correlated to day 2 embryo quality. FF Leptin and Activin A did not correlate to day 2 embryo qualities or pregnancy outcome.
FF total Activin A level did not relate to FF Leptin level or number of oocytes collected. Low or high FF Activin A or Leptin levels did not show an effect on fertilization, pregnancy or day 2 embryo qualities.
Conclusion: Neither FF Activin A nor FF Leptin levels can be used to predict IVF outcome measures.