Four Generations of Sequencing- Is it Ready for the Clinic Yet?
Srilakshmi Srinivasan and Jyotsna Batra*
Australian Prostate Cancer Centre- Queensland, Translational Research Institute, Queensland University of Technology, Woolloongabba, AustraliaSanger
- *Corresponding Author:
- Jyotsna Batra
Australian Prostate Cancer Centre- Queensland
Translational Research Institute
Queensland University of Technology
Woolloongabba, Queensland, Australia
Tel: +61 7 3443 7336
Fax: +61 7 34437336
E-mail: [email protected]
Received date: January 27, 2014; Accepted date: July 11, 2014; Published date: July 17, 2014
Citation: Srinivasan S, Batra J (2014) Four Generations of Sequencing- Is it Ready for the Clinic Yet?. Next Generat Sequenc & Applic 1:107. doi:10.4172/2469-9853.1000107
Copyright: © 2014 Srinivasan S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Next-generation sequencing techniques have revolutionized over the last decade providing researchers with low cost, high-throughput alternatives compared to the traditional Sanger sequencing methods. These sequencing techniques have rapidly evolved from first-generation to fourth-generation with very broad applications such as unraveling the complexity of the genome, in terms of genetic variations, and having a high impact on the biological field. In this review, we discuss the transition of sequencing from the second-generation to the third- and fourthgenerations, and describe some of their novel biological applications. With the advancement in technology, the earlier challenges of minimal size of the instrument, flexibility of throughput, ease of data analysis and short run times are being addressed. However, the need for prospective analysis and effectiveness to test whether the knowledge of any given new variants identified has an effect on clinical outcome may need improvement.