alexa Frequency of NRAMP1 Gene Polymorphisms among Canadian First Nations Peoples Experiencing Endemic Tuberculosis | OMICS International | Abstract
ISSN: 2161-1068

Mycobacterial Diseases
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Research Article

Frequency of NRAMP1 Gene Polymorphisms among Canadian First Nations Peoples Experiencing Endemic Tuberculosis

Linda A Larcombe1,2,3a*, Neeloffer Mookherjee1,3c, Andrew M Lodge4b, Jodie S Brown5b, Lizette Denechezhe5b, Peter W Nickerson1,3a and Pamela H Orr1,2,3a

1Department of Internal Medicine, Faculty of Health Sciences, College of Medicine, University of Manitoba, Canada

2Department of Medical Microbiology and Infectious Diseases, Faculty of Health Sciences, College of Medicine, University of Manitoba, Canada

3Department of Community Health Sciences, Faculty of Health Sciences, College of Medicine, University of Manitoba, Canada

4Manitoba Centre for Proteomics and Systems Biology, Faculty of Health Sciences, College of Medicine, University of Manitoba, Canada

5Northlands Denesuline First Nation, Lac Brochet, Manitoba, Canada

aThese authors contributed to the research design, data analysis and the manuscript preparation

bThese authors contributed to the data collection

cThese authors contributed to the preparation of the manuscript

Corresponding Author:
Linda Larcombe
Department of Internal Medicine
Faculty of Health Sciences
College of Medicine, University of Manitoba, Canada
Tel: 1-204-975-7726
E-mail: [email protected]

Received Date: July 31, 2015; Accepted Date: September 28, 2015; Published Date: October 5, 2015

Citation: Larcombe LA, Mookherjee N, Lodge AM, Brown JS, Denechezhe L, et al. (2015) Frequency of NRAMP1 Gene Polymorphisms among Canadian First Nations Peoples Experiencing Endemic Tuberculosis. Mycobact Dis 5:193. doi:10.4172/2161-1068.1000193

Copyright: © 2015 Larcombe LA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Objectives: The natural resistance-associated macrophage protein 1 (NRAMP1) regulates susceptibility to infectious and autoimmune diseases. NRAMP1 gene polymorphisms have been implicated in susceptibility to tuberculosis. The frequency of NRAMP1 gene polymorphisms was therefore evaluated in three Manitoba First Nations sub-groups (Dene, Cree, and Saulteaux) with differential but high rates of tuberculosis (636/100,000, 496/100,000, and 0/100,000 respectively).

Methods: Venous blood samples were collected from 281 study participants from three First Nations sub-groups (Dene (N=108), Cree (N=41), Saulteaux (N=49)), and a non-indigenous Canadian-born (European-descent) group (N=83). Genomic DNA was extracted and four single nucleotide polymorphisms in the NRAMP1 gene (5′ (GT)n, -274 (C/T), Intron 4 (469+14 G/C), D543N (G/A)) were genotyped using restriction fragment length polymorphism. NRAMP1 SNP allele frequencies were counted and compared between studied sub-groups.

Results: The Dene sub-group had significantly different allele frequencies of NRAMP1 (5′(GT)n, -274 (C/T), Intron 4(G/C), D543N (G/A)) compared to the European-descent group. The NRAMP1 allele frequencies at D543N (G/A) and Intron 4(G/C) differed significantly between the Cree and the European-descent group while the allele frequencies of the Saulteaux were not significantly different from the European-descent group. Two sub-groups (Dene and Cree) had higher frequency of NRAMP1 D543N (A) allele, which is associated with tuberculosis in other populations.

Conclusion: High, but differential rates of tuberculosis among the First Nation sub-groups in Manitoba are related to social determinants of health (i.e. poverty, racism, inadequate housing) but other potential risk factors such as gene polymorphisms associated with tuberculosis have only recently come under investigation. NRAMP1 allele frequencies were found to be different comparing the Dene, Cree and Saulteaux and their role in tuberculosis susceptibility/resistance needs further investigation.

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