FreundÃ¢ÂÂs Complete Adjuvant (FCA) Could Reverse the Depressive-Like Symptoms Induced by Chronic Mild Stress (CMS) in MiceHacene Frih1*, Lamia Bensaci1, Redha Djenidi1, Bachir Ali Rachedi1, Tahraoui Abdelkrim1 and Sylvain Fisson2
- Corresponding Author:
- Hacene FRIH
faculté des Sciences
Département de Biologie
Laboratory of Applied Neuro-Endocrinology
Université Badji Mokhtar Annaba, Algeria
E-mail: [email protected]
Received date: December 12, 2012; Accepted date: January 16, 2013; Published date: January 20, 2013
Citation: Frih H, Bensaci L, Djenidi R, Rachedi BA, Fisson S (2013) Freund’s Complete Adjuvant (FCA) Could Reverse the Depressive-Like Symptoms Induced by Chronic Mild Stress (CMS) in Mice. J Neurol Neurophysiol 4:143. doi:10.4172/2155-9562.1000143
Copyright: © 2013 Frih H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: The objective of this work was a comparative study of two animal models in female mice: Chronic Mild Stress (CMS) and Chronic Mild Stress combined with chronic inflammation with complete Freund’s adjuvant (CMS+CFA). Methods: Mice were exposed to CMS for 40 days as food deprivation, water deprivation, forced swimming, flashing light, isolation, wet bedding and wet litter at 4°C. The stress was applied at times that change every day, in order to minimize its predictability. A subcutaneous injection of 0.03 mg/ml of CFA was administered to CMS groups the 18th day of experimentation. Recordings of sequences of behavior in elevated plus maze, open field test and water maze test was made. After decapitation, the brain, thymus, spleen, liver, kidneys, adrenals and ovaries were weighed and we calculated their relative weights. Results: Exposure of mice to CMS caused a depraessive-like behavior revealed by behavioral tests, accompanied by a decreased gain of body weight, locomotor activity and impaired spatial memory signaled by the Morris water maze test. The injection of CFA in mice submitted to a CMS has provided a partial repair of the memory in the Morris water maze, locomotor activity, gain of body weight, weight of adrenals. Conclusion: Freund’s complete adjuvant (FCA) could reverse the depressive-like symptoms induced by chronic mild stress (CMS) in mice.