alexa FTIR has the Potential to Detect Stem Cells in the Bovine Corneal Stroma
ISSN: 2161-0398

Journal of Physical Chemistry & Biophysics
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Short Communication

FTIR has the Potential to Detect Stem Cells in the Bovine Corneal Stroma

Christina S. Kamma-Lorger1*, Katia Wehbe2, Craig Boote1, Keith M. Meek1 and Gianfelice Cinque2
1Structural Biophysics Group, School of Optometry and Vision Sciences, Cardiff University CF24 4LU, UK
2Diamond Light Source, Didcot, Oxfordshire OX11 0DE
Corresponding Author : Christina S. Kamma-Lorger
Structural Biophysics Group
School of Optometry and Vision Sciences
Cardiff University CF24 4LU, UK
E-mail: [email protected]
Received November 07, 2011; Accepted December 26, 2011; Published December 29, 2011
Citation: Kamma-Lorger CS, Wehbe K, Boote C, Meek KM, Cinque G (2011) FTIR has the Potential to Detect Stem Cells in the Bovine Corneal Stroma. J Physic Chem Biophysic 1:103. doi: 10.4172/2161-0398.1000103
Copyright: © 2011 Gatenby RA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Fourier Transform Infrared Spectroscopy (FTIR) was used for the first time to locate stem cells in the corneal stroma. An off-line microscope was used in Diamond light source and a bovine cryo-section was examined. Differences in spectra from two different regions suggest the existence of a cell population that has stem cell characteristics. Synchrotron FTIR would provide a greater scanning resolution than the off-line microscope used, therefore it could be an ideal technique to locate stromal stem cells in the cornea.

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