alexa Functional Recovery of a Whole Ovary Transplanted Into Syngenic Testis in Mice
ISSN: 2168-9849

Cloning & Transgenesis
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Research Article

Functional Recovery of a Whole Ovary Transplanted Into Syngenic Testis in Mice

Masahiro Sato1*, Masato Ohtsuka2, Shingo Nakamura3, Takayuki Sakurai4, Satoshi Watanabe5 and Yukiko Yasuoka6
1Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Japan
2Division of Basic Molecular Science and Molecular Medicine, School of Medicine, Tokai University, Kanagawa, Japan
3Department of Surgery, National Defense Medical College, Saitama, Japan
4Department of Organ Regeneration, Graduate School of Medicine, Shinshu University, Nagano, Japan
5Animal Genome Research Unit, Division of Animal Science, National Institute of Agrobiological Sciences, Ibaraki, Japan
6Department of Physiology, Kitasato University, School of Medicine, Kanagawa, Japan
Corresponding Author : Masahiro Sato
Section of Gene Expression Regulation
Frontier Science Research Center
Kagoshima University, Kagoshima, Japan
Tel: 81-99-285-3585
Fax: 81-99-285-3585
E-mail: [email protected]
Received June 05, 2012; Accepted July 12, 2012; Published July 16, 2012
Citation: Sato M, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S (2012) Functional Recovery of a Whole Ovary Transplanted Into Syngenic Testis in Mice. Clon Transgen 1:102. doi:10.4172/2168-9849.1000102
Copyright: © 2012 Sato M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

We previously demonstrated that a whole juvenile (2-week-old) mouse ovary transplanted into a syngenic host testis survived and exhibited normal folliculogenesis. The aim of this study was to test whether the graft possessed normal abilities such as ovulation. Whole ovaries were excised from a female transgenic mouse (2-week-old) ubiquitously expressing enhanced green fluorescence protein. The isolated ovary was then transplanted into the testis of a nontransgenic male (2-week-old), in which approximately 80% of the seminiferous tubules had been removed to create enough space for the graft to grow properly. Two months after transplantation, the ovary grafts were isolated from the testis and subjected to histological analysis. Some grafts were subsequently subjected to ovary transplantation to assess the capability of the grafted ovaries to ovulate oocytes. Two months after ovary transplantation, 3 of 8 ovaries grafted under the ovarian capsule successfully ovulated fluorescent oocytes in response to a surge of luteinizing hormone. These results indicate that intratesticular grafting of juvenile ovaries allows functional development of the graft. This system would be helpful for investigating oogenesis/folliculogenesis in ectopic environments as well as for rescuing ovaries with genetic defects.

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