Fundulopanchax gardneri Test: A Convenient Method of Bioassay for Active Constituents of Natural Products
|Ado K, Garba S* and Salihu L|
|Department of Chemistry, Nigerian Defence Academy, Kaduna, Nigeria|
|Corresponding Author :||Garba S
Department of Chemistry
Nigerian Defence Academy, Kaduna, Nigeria
E-mail: [email protected]
|Received March 19, 2014; Accepted April 26, 2014; Published April 28, 2014|
|Citation: Ado K, Garba S, Salihu L (2014) Fundulopanchax gardneri Test: A Convenient Method of Bioassay for Active Constituents of Natural Products. Nat Prod Chem Res 2:133. doi:10.4172/2329-6836.1000133|
|Copyright: © 2014 Ado K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Aim: To apply the Brine Shrimp Lethality Test (BST) procedure on locally available Fundulopanchax gadneri killifish with a view to achieving the same result.
Study design: Extraction of crude extracts from plants, evaluation of the bioactivity of the extracts using brine shrimp and Fundulopanchax gadneri killifish. Statistical analysis of the results obtained.
Place and duration of the study: Sample: Afaka, Kaduna north local government, Kaduna State, Nigeria. Department of Chemistry, Nigerian Defence Academy, Kaduna. Nigeria, between June 2011 and August 2013.
Methodology: Batch method of extraction, brine shrimp lethality test as adopted by Meyer et al., 1982 using brine shrimp and Fundulopanchax gadneri killifish.
Results: The results of the brine shrimp and Fundulopanchax gadneri killifish bioassay indicate that eleven out of the thirteen medicinal plants screened for bioactivity were active. Statistical analysis of the results showed a correlation coefficient of 0.970, 0.99 and 0.96 respectively for both BST and FGT suggesting that there is a positive correlation between the two variables.
Conclusion: The results are in consonance suggesting that Fundulopanchax gadneri killifish test can be used as a preliminary method of assessing the cytotoxicity of natural products.