alexa Future Prospects of 3D Human Chromosome Imaging by Serial Block Face Scanning Electron Microscopy | OMICS International
ISSN: 2168-9431

Single Cell Biology
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Commentary

Future Prospects of 3D Human Chromosome Imaging by Serial Block Face Scanning Electron Microscopy

Mohammed Yusuf1,2* Bo Chen1,2 and Ian Robinson1,2
1London Centre for Nanotechnology, University College London, London, UK
2Research Complex at Harwell, Rutherford Appleton Laboratory, Oxon, UK
*Corresponding Author : Mohammed Yusuf
London Centre for Nanotechnology
University College London, London
Research Complex at Harwell
Rutherford Appleton Laboratory, Oxon, UK
Tel: +44 20 7679 2000
E-mail: [email protected]
Received: March 14, 2016 Accepted: April 05, 2016 Published: April 07, 2016
Citation: Yusuf M, Chen B, Robinson I (2016) Future Prospects of 3D Human Chromosome Imaging by Serial Block Face Scanning Electron Microscopy. Single Cell Biol 5:134. doi:10.4172/2168-9431.1000134
Copyright: © 2016 Yusuf M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The higher order structure of human chromosomes remains to be elucidated with the 30 nm mystery still remaining. For internal structure determination, transmission electron microscopy (TEM) cannot be used as the chromosomes are too thick (approx. 1.4 microns) and scanning electron microscopy (SEM) is a surface imaging technique. For this purpose, the three-dimensional (3D) serial block face scanning electron microscopy (SBFSEM) was used on imaging mitotic human chromosome for the first time [1].

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