Gas Chromatographic Determination of Prochloraz in Ten Herbal Medicines
- *Corresponding Author:
- Dr. Jialun Wu
Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects
Institute of Pesticide and Environmental Toxicology
Zhejiang University, Hangzhou 310029, China
Tel: 0086 0571 86971221
Fax: 0086 0571 86971221
E-mail: [email protected]
Received date: May 25, 2011; Accepted date: July 19, 2011; Published date: July 21, 2011
Citation: Wu J, Zhao J, Wang T, Chen A, Xue J (2011) Gas Chromatographic Determination of Prochloraz in Ten Herbal Medicines. Pharm Anal Acta 2:128. doi: 10.4172/2153-2435.1000128
Copyright: © 2011 Wu J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A method for determining prochloraz in ten herbal medicines is described. Pesticide standards were fortified into herbal medicines at 3 levels (0.005, 0.01 and 0.05mg/kg). Prochloraz reacts with pyridine hydrochloride and generates a hydrolysate, the 2,4,6-trichlorophenol which responded intensively in GC-ECD. The results showed average recoveries were between 76.6% and 105.1%. The method evidenced good accuracy and precision for monitoring prochloraz in ten herbal medicine samples. The limit of quantification (LOQ) was 0.005mg/kg. It is linear from 0.005 mg/L to 2.000mg/L. The regression equation is y=3816.1x-7.7835, R=0.9997.