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Gas Signatures from Cultured Neutrophils and Peripheral Blood Mononuclear Cells Obtained from Healthy Humans | OMICS International | Abstract

Journal of Molecular Biomarkers & Diagnosis
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Research Article

Gas Signatures from Cultured Neutrophils and Peripheral Blood Mononuclear Cells Obtained from Healthy Humans

Hye-Won Shin*,#, Brandon J. Umber#, Simone Meinardi, Szu-Yun Leu, Frank Zaldivar, Donald R. Blake# and Dan M. Cooper

Department of Pediatrics University of California, Irvine, Irvine, CA 92697

#Contributed Equally

*Corresponding Author:
Hye-Won Shin, PhD
Department of Pediatrics
1303A Hewitt Hall, University of California
Irvine, CA 92697
Tel: 949-824-3702
Fax: 949-824-336
E-mail: [email protected]

Received Date: June 08 Accepted Date: August 22, 2011; Published Date: August 26, 2011

Citation: Shin H, Umber BJ, Meinardi S, Leu S, Zaldivar F, et al. (2011) Gas Signatures from Cultured Neutrophils and Peripheral Blood Mononuclear Cells Obtained from Healthy Humans. J Mol Biomark Diagn 2:112. doi:10.4172/2155-9929.1000112

Copyright: © 2011 Shin H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited


We hypothesized that leucocytes may be a significant source of physiologically active gases. Furthermore, it is hoped that alterations by disease of these volatile organic compound (VOC) profiles can ultimately be used as noninvasive biomarkers. We recently demonstrated that transformed promyelocitic cells produce distinct gases. (J Transl Med. 2009 7:31). The current study extends this work to neutrophils and peripheral blood mononuclear cells (PBMCs). Cells were isolated from the peripheral blood of healthy donors (n=10, 18-65 yrs old), resuspended in RPMI, and incubated in bioreactors for 24 hrs. The headspace was analyzed using gas chromatography. Acetaldehyde was elevated from neutrophils(median (min, max); 197 (34, 577) ppbv) compared with media (88 (40,116) ppbv, p=0.014). In the presence of alanine, neutrophils emitted more acetaldehyde (>1.5 fold compared to basal level). In contrast, acetaldehyde from PBMCs was 26 (8, 89) ppbv, significantly below media (p=0.004). Adding alanine did not affect acetaldehyde emissions from PBMCs. Also, hexanaldehyde appeared metabolized by neutrophils and PBMCs. This study demonstrates 1) that human primary immune cells produce measurable VOCs in vitro, and 2) the ability to detect basal levels of acetaldehyde from unperturbed cultured neutrophils. Moreover, the data suggest that different leukocyte subtypes have different VOC profiles.


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