GC-MS Analysis and Phytochemical Screening of A Rare Pteridophyte Nephrolepis Cardifolia (L.) Presl. from Tirumala Hills
Plants have been an important source of medicine with qualities for thousands of years. Mainly on traditional remedies such as ferns for their history it has been used as a popular folk medicine. Nephrolepis cardifolia(L.) Presl. of family Nephrolepidaceae found in hilly area of Tirumala hills, Tirupathi, Andhra Pradesh, India. Ethanomedicinally, the genus is important and popularly known as Fishbone fern. The present paper reveals the phytochemical screening and GC-MS analysis of Nephrolepis cardifolia. Preliminary phytochemical studies showed the presence of alkaloids, flavonoids, phenols, tannins and saponins in higher to moderate concentrations. Acetone extract of the plant was analyzed using Gas Chromatography-Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technologies (NIST) library. Gas chromatography mass spectrometry (GC-MS) analysis revealed the presence of six compounds. The phytoconstituents screened were Neophytadiene and 2,6,10-trimethyl,14,ethylene,2 Hexadecen-1-ol and 3,7,11,15 tetramethyl R-R, hexadecanoic acid & palmitic acid, 9 octadecenoic acid(z) & oleic acid, octadecanoic acid & stearic acid,Stigmast-4-en-3-one & 4-Stigmasten-3-one. The six compounds were identified by comparing their retention time and peak area with that of literature and by interpretation of mass spectra. Hence, at any rate Nephrolepis cardifolia is an attractive material for further research leading to possible drug development.