alexa Gel Filtration Chromatography Technique as Tool of Simple Study SeminalPlasma Proteins in Domestic Animals | OMICS International | Abstract
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Gel Filtration Chromatography Technique as Tool of Simple Study SeminalPlasma Proteins in Domestic Animals

Vasconcelos André Belico de*, Oliveira Jamil Silvano de, Lagares Monique de Albuquerque

University of Uberaba, Av. Nenê Sabino 1800, Uberaba, Minas Gerais, Brazil

*Corresponding Author:
Vasconcelos André Belico de
University of Uberaba
Av. Nenê Sabino 1800, Uberaba
Minas Gerais, Brazil, CEP 38061-500
Tel: +55-34-88120157
Fax: +55-34-33148910
E-mail: [email protected], [email protected]

Received date: July 03, 2015; Accepted date: July 20, 2015; Published date: July 27, 2015

Citation: Belico de VA, Silvano de OJ, de Albuquerque LM (2015) Gel Filtration Chromatography Technique as Tool of Simple Study Seminal Plasma Proteins in Domestic Animals. J Chromatogr Sep Tech 6:281. doi:10.4172/2157- 7064.1000281

Copyright: © 2015 Belico de VA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Seminal fluid is the liquid component of sperm, providing a safe surrounding for spermatozoa. The seminal plasma has the feature common to many other body fluid, characterized by a high dynamic range of proteins, which visualizes physiological and biochemical processes of semen. As in other body fluids, it is convenient to distinguish in the seminal plasma between proteins and non-proteins. The molecular exclusion chromatographic technique presents important points to constitutional proteins preservation, in addition to the conventional phenomenon; witch exclusion hydrophobic interactions can provide a higher resolution in the chromatogram and do not provide nonspecific interactions between protein structures. The aim was the chromatography profile to simple study of proteins seminal plasma domestic animals (Stallions, Canine, and Goat), by the technique of gel filtration chromatography. The samples (seminal plasma proteins) were chromatographed in a Superose 12 HR 10/30, equilibrated with 25 mMTris-HCl (Sigma) with 0.15 M NaCl (Sigma), pH 7.4 at room temperature in a fast performance liquid chromatography (FPLC-system), using a flow rate of 0.5 mL.min-1. There was a molecular separation of significantly different molecular weights, which makes possible the logarithmic relationship between molecular weight and the elution volume. Calibration of the gel filtration column resulted in an equation y = ax + b, where the values of a and b were 5.43 and -2.175, respectively, and the corresponding errors were 0.115 and 0.256, respectively. The experimental error was less than 5% for most of the protein molecular masses. The work showed that the gel filtration chromatography technique provided an excellent analytical repeatability, and could therefore, be a valuable tool to the study preliminary of seminal plasma.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version