Gel-based Proteomic Characterization of Soluble and Insoluble Fraction Proteins in Rat Spinal Cord
- *Corresponding Author:
- Professor Shaojun Liu
State Key Laboratory of Proteomics and Department of Neurobiology
Institute of Basic Medical Sciences
Beijing, 100850, P.R.China
E-mail: [email protected]
Received Date: January 22, 2010; Accepted Date: February 23, 2010; Published Date: February 23, 2010
Citation: Yang S, Ding Q, Guo Y, Zhao C, Jia Y, et al. (2010) Gel-based Proteomic Characterization of Soluble and Insoluble Fraction Proteins in Rat Spinal Cord. J Proteomics Bioinform 3: 074-081. doi: 10.4172/jpb.1000124
Copyright: © 2010 Yang S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permitsunrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Fractionation efficiency and protein characterization of neural soluble and insoluble proteins by sequential extraction was scrutinized by gel-based proteomic analysis. Spinal cord proteins of adult rats were first extracted with aqueous buffer (fraction A), followed by standard (fraction B) or modified (fraction C) enhanced solutions. Of the top 30 most abundant proteins in fractions A, B and C, the percentage of cytoplasmic proteins was 74% (28/38) , 37% (14/38) and 42% (15/36), respectively; membrane organellar proteins accounted for 8% (3/38), 45% (17/38), and 44% (16/36); membrane proteins accounted for 13% (5/38), 18% (7/38) and 14% (5/36). The number of hydrophobic domains was 5, 15 and 9. Shared proteins in three fractions were only 13%. When additional less abundant 30 spots enriched the insoluble fraction C were characterized, membrane proteins accounted for 31%, among which 83% were peripheral membrane proteins and 17% were integral membrane proteins. Functional analysis also revealed some difference between different fractions although all fractionated proteins are involved in energy metabolism, redox regulation, signal transduction and cellular architecture.