alexa Gene Expression Profiling from a Prostate Cancer PC-3 C
ISSN: 1948-5956

Journal of Cancer Science & Therapy
Open Access

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Research Article

Gene Expression Profiling from a Prostate Cancer PC-3 Cell Line Treated with Salinomycin Predicts Cell Cycle Arrest and Endoplasmic Reticulum Stress

Kwang-Youn Kim1#, Young-Kyo Seo2#, Sun-Nyoung Yu1, Sang-Hun Kim1, Pann-Ghill Suh2, Jae-Hoon Ji3, Hak-Sun Yu4, Yeong-Min Park1 and Soon-Cheol Ahn1,5*

1Department of Microbiology & Immunology, Pusan National University School of Medicine, Yangsan 626-870, Republic of Korea

2School of NanoBioscience and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan 689-798, Republic of Korea

3Genome Instability Research Center, Ajou University School of Medicine, Suwon 443-721, Republic of Korea

4Department of Parasitology, Pusan National University School of Medicine, Yangsan 626-870, Republic of Korea

5Medical Research Institute, Pusan National University, Yangsan 626-870, Republic of Korea

#These authors contributed equally to this work

*Corresponding Author:
Soon-Cheol Ahn
Professor
Department of Microbiology & Immunology
Pusan National University School of Medicine
Yangsan 626-870, Republic of Korea
Tel: +82-51-510-8092
Fax: +82-55-382- 8090
E-mail: [email protected]

Received date: November 01, 2012; Accepted date: December 05, 2012; Published December 07, 2012

Citation:Kim KY, Seo YK, Yu SN, Kim SH, Suh PG, et al. (2013) Gene Expression Profiling from a Prostate Cancer PC-3 Cell Line Treated with Salinomycin Predicts Cell Cycle Arrest and Endoplasmic Reticulum Stress. J Cancer Sci Ther 5: 023-030.. doi: 10.4172/1948-5956.1000180

Copyright: © 2013 Kim KY, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Abstract
Previously, we reported that salinomycin induces apoptosis of human prostate cancer cells through accumulated reactive oxygen species and mitochondrial membrane depolarization. To extend our understanding for the genomewide expression pattern, we performed cDNA microarray analysis for gene expression profiles in prostate PC-3 cells treated with salinomycin. We found a couple of differences from gene expression profiles. First of them, cyclins and cyclin-dependent kinases were down-regulated, whereas cyclin dependent kinase inhibitors were upregulated, implicating inhibition of cell cycle progression. Second, HSPA5/Bip, DDIT3/CHOP, TRIB3, ATF4 and ATF6 regarding endoplasmic reticulum (ER) stress and unfolded protein response (UPR) were increased at mRNA and protein levels, indicating salinomycin-induced growth inhibition of PC-3 cells seem to be mediated through induction of ER stress and activation of the UPR pathway. Our finding should be useful for understanding genomewide expression patterns of salinomycin-mediated cell cycle arrest and ER stress response toward induction of apoptosis and be helpful for finding future cancer therapeutic targets in prostate cancer cells.

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