Generation of a Ustilago maydis ade2 Mutant
- *Corresponding Author:
- Jakob H Waterborg
Division of Cell Biology and Biophysics, School of Biological Sciences
University of Missouri-Kansas City, Kansas City, MO, USA
E-mail: [email protected]
Received date: Apr 29, 2016; Accepted date: May 23, 2016; Published date: May 30, 2016
Citation: Verma A, Kapros T, Waterborg JH (2016) Generation of a Ustilago maydis ade2 Mutant. Fungal Genom Biol 6:138. doi:10.4172/2165-8056.1000138
Copyright: © 2016 Verma A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The need exists to create the auxotrophic mutants for basidiomycete Ustilago maydis to allow selection of gene transformants in minimal growth media. The ADE2 gene was identified by homology with Saccharomyces cerevisiae. Adenine-requiring auxotrophic mutants were effectively created by homologous recombination in protoplasts using a standard plasmid containing the ADE2 locus interrupted by a phleomycin resistance gene, selected on standard complex media. The knockout ade2 mutant produced grows on minimal, defined medium only if supplemented with adenine.