alexa Generation of a Ustilago maydis ade2 Mutant
ISSN: 2165-8056

Fungal Genomics & Biology
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Generation of a Ustilago maydis ade2 Mutant

Anju Verma1, Tamas Kapros2 and Jakob H Waterborg2*

1Division of Plant Sciences and Bond Life Sciences Center, University of Missouri, Columbia, MO, USA

2Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO, USA

*Corresponding Author:
Jakob H Waterborg
Division of Cell Biology and Biophysics, School of Biological Sciences
University of Missouri-Kansas City, Kansas City, MO, USA
Tel: 1-913-219-5414
E-mail: [email protected]

Received date: Apr 29, 2016; Accepted date: May 23, 2016; Published date: May 30, 2016

Citation: Verma A, Kapros T, Waterborg JH (2016) Generation of a Ustilago maydis ade2 Mutant. Fungal Genom Biol 6:138. doi:10.4172/2165-8056.1000138

Copyright: © 2016 Verma A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



The need exists to create the auxotrophic mutants for basidiomycete Ustilago maydis to allow selection of gene transformants in minimal growth media. The ADE2 gene was identified by homology with Saccharomyces cerevisiae. Adenine-requiring auxotrophic mutants were effectively created by homologous recombination in protoplasts using a standard plasmid containing the ADE2 locus interrupted by a phleomycin resistance gene, selected on standard complex media. The knockout ade2 mutant produced grows on minimal, defined medium only if supplemented with adenine.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version