Generations of Sequencing Technologies: From First to Next GenerationMehdi Kchouk1,3*, Jean-François Gibrat2 and Mourad Elloumi3
- *Corresponding Author:
- Mehdi Kchouk
Faculty of Sciences of Tunis (FST)
Tunis El-Manar University, Tunisia
Tel: +216 71 872 253
E-mail: [email protected]
Received Date: January 31, 2017; Accepted Date: February 27, 2017; Published Date: March 06, 2017
Citation: Kchouk M, Gibrat JF, Elloumi M (2017) Generations of Sequencing Technologies: From First to Next Generation. Biol Med (Aligarh) 9: 395. doi:10.4172/0974-8369.1000395
Copyright: © 2017 Kchouk M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
DNA sequencing process utilizes biochemical methods in order to determine the correct order of nucleotide bases in a DNA macromolecule using sequencing machines. Ten years ago, sequencing was based on a single type of sequencing that is Sanger sequencing. In 2005, Next Generation Sequencing Technologies emerged and changed the view of the analysis and understanding of living beings. Over the last decade, considerable progress has been made on new sequencing machines. In this paper, we present a non-exhaustive overview of the sequencing technologies by beginning with the first methods history used by the commonly used NGS platforms until today. Our goal is to provide beginners in the field as well as to the amateurs of science a simple and understandable description of NGS technologies in order to provide them with basic knowledge as an initiation into this field in full ardor.