alexa Generations of Sequencing Technologies: From First to Next Generation
ISSN: 0974-8369

Biology and Medicine
Open Access

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Review Article

Generations of Sequencing Technologies: From First to Next Generation

Mehdi Kchouk1,3*, Jean-François Gibrat2 and Mourad Elloumi3

1Faculty of Sciences of Tunis (FST), Tunis El-Manar University, Tunisia

2Research Unit Applied Mathematics and Computer Science, from Genomes to the Environment' (MaIAGE), Jouy en Josas, France

3Laboratory of Technologies of Information and Communication and Electrical Engineering, National Superior School of Engineers of Tunis, University of Tunis, Tunisia

*Corresponding Author:
Mehdi Kchouk
Faculty of Sciences of Tunis (FST)
Tunis El-Manar University, Tunisia
Tel: +216 71 872 253
E-mail: [email protected]

Received Date: January 31, 2017; Accepted Date: February 27, 2017; Published Date: March 06, 2017

Citation: Kchouk M, Gibrat JF, Elloumi M (2017) Generations of Sequencing Technologies: From First to Next Generation. Biol Med (Aligarh) 9: 395. doi:10.4172/0974-8369.1000395

Copyright: © 2017 Kchouk M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

 

Abstract

DNA sequencing process utilizes biochemical methods in order to determine the correct order of nucleotide bases in a DNA macromolecule using sequencing machines. Ten years ago, sequencing was based on a single type of sequencing that is Sanger sequencing. In 2005, Next Generation Sequencing Technologies emerged and changed the view of the analysis and understanding of living beings. Over the last decade, considerable progress has been made on new sequencing machines. In this paper, we present a non-exhaustive overview of the sequencing technologies by beginning with the first methods history used by the commonly used NGS platforms until today. Our goal is to provide beginners in the field as well as to the amateurs of science a simple and understandable description of NGS technologies in order to provide them with basic knowledge as an initiation into this field in full ardor.

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