Genetic and Pathogenic Variability of Ascochyta rabiei Isolates from Pakistan and Syria as Detected by Universal Rice Primers
Hina Ali*, Syed Sarwar Alam and Nayyer Iqbal
Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan
- *Corresponding Author:
- Hina Ali
Nuclear Institute for Agriculture and Biology
E-mail: [email protected]hotmail.com
Received date: November 25, 2013; Accepted date: December 24, 2013; Published date: December 28, 2013
Citation: Ali H, Alam SS, Iqbal N (2013) Genetic and Pathogenic Variability of Ascochyta rabiei Isolates from Pakistan and Syria as Detected by Universal Rice Primers. J Plant Pathol Microb 4: 212 doi:10.4172/2157-7471.1000212
Copyright: © 2013 Ali H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Ascochyta rabiei is the casual agent of blight disease of chickpea (Cicer arietinum L.). The study was aimed to assess the genetic diversity of highly aggressive Ascochyta rabiei (AR) isolates (pathotypes III and IV) from Syria and its comparison with highly aggressive isolates from Pakistan. AR isolates were characterized for pathogenicity assay and genetic variability. Previously genetic variability of AR isolates have been checked with RAPD and SSR markers, here we are reporting for the first time diversity using ten Universal rice primers (URP) derived from the repeat sequence of rice genome. URP proved very useful for the characterization of isolates and clearly differentiated Syrian pathotypes from Pakistani ones. URP can be helpful in studying the population variability from AR pathotypes worldwide.