Genetic Determination of Potential Trichoderma Species Using ISSR (Microsatellite) Marker in Uttar Pradesh, India
Mohammad Shahid, Mukesh Srivastava, Vipul Kumar*, Anuradha Singh and Sonika Pandey
Department of Plant Pathology, Biocontrol Laboratory, C. S. A. University of Agriculture & Technology, India
- *Corresponding Author:
- Mohammad Shahid
Department of Plant Pathology
C. S. A. University of Agriculture & Technology
Tel: 0512 253 4156
E-mail: [email protected]
Received date: February 17, 2014; Accepted date: April 10, 2014; Published date: April 16, 2014
Citation: Shahid M, Srivastava M, Kumar V, Singh A, Pandey S (2014) Genetic Determination of Potential Trichoderma Species Using ISSR (Microsatellite) Marker in Uttar Pradesh, India. J Microb Biochem Technol 6:174-178. doi: 10.4172/1948-5948.1000139
Copyright: © 2014 Shahid M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Seven Trichoderma sp. were collected from different locations of Uttar Pradesh, India for evaluating their bioefficiency by determining their genetic variations. PCR-based Inter Simple Sequence Regions (ISSR) Marker employing 6 primers produced 30 scorable bands out of which 27 bands were polymorphic. The Unweighted Pair Group Method of Arithmetic Means (UPGMA) dendrogram constructed from Nei’s  genetic distance produced 2 main clusters (1 isolates in cluster 1 and 6 isolates in cluster 2). The result indicating their genetic diversity has opened new possibility of using the most efficient and more isolates of Trichoderma in the preparation of effective biopesticide.