GENETIC DISCRIMINATION OF TWO COWPEA (VIGNA UNGUICULATA (L.) WALP) BRUCHID (COLEOPTERA, CHRYSOMELIDAE, BRUCHINAE): CALLOSOBRUCHUS MACULATUS (F.) AND BRUCHIDIUS ATROLINEATUS (PIC.)
Awa Ndiaye1,2, Philippe Gauthier3 and Mbacké Sembène1
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The cowpea, Vigna unguiculata (L.) Walp is a food leguminous plant cultivated in tropical Africa for its seeds rich in proteins. The main problem for its production is the conservation of the harvests. In the fields as in the stocks, the seeds are destroyed by pests (bruchids). Bruchidius atrolineatus Pic. and Callosobruchus maculatus Fab. (Coleoptera- Bruchidae) are the most important pests of cowpea beans. Cowpea infestation by these two bruchid species starts in the field when the plant begins to bear fruit and continues through storage, resulting in substantial damage if no control action is taken, they can destroy 80 to 100% crop after several months of storage. The objective of this work is to develop a fast and reliable identification method of these species of Bruchidae through PCR-RFLP. Primary outcomes were recorded allow to consider that portion of DNA amplified with our primer pairs has no restriction site for the enzyme BsmAI in Bruchudius atrolineatus both cytochrome b and for the 28S. For Callosobruchus maculatus with the same restriction enzyme, DNA is cut into 3 fragments for the cytochrome b gene and 2 for the 28S.