Genetic Diversity Analysis in Chickpea Employing ISSR MarkersGautam AK1, Gupta N1, Bhadkariya R1, Srivastava N2 and Bhagyawant SS1*
- Corresponding Author:
- Bhagyawant SS
School of Studies in Biotechnology, Jiwaji University, Gwalior, 474011, India
Tel: 09691675884, 07512442705
E-mail: [email protected]
Received Date: October 18, 2016; Accepted Date: November 16, 2016; Published Date: November 23, 2016
Citation: Gautam AK, Gupta N, Bhadkariya R, Srivastava N, Bhagyawant SS (2016) Genetic Diversity Analysis in Chickpea Employing ISSR Markers. Agrotechnology 5:152. doi: 10.4172/2168-9881.1000152
Copyright: © 2016 Gautam AK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In the present study, inter simple sequence repeat (ISSR) markers were employed to estimate genetic diversity in 13 accessions of chickpea including cultivated and wild. Among all these anchored ISSR primers tested, pentanucleotide repeat primer UBC-879 produced better amplification patterns. A total of 150 bands were amplified in a molecular weight range of 100-2000 bps revealing an average of 21.4 bands per primers and 1.64 bands per primer per genotype. The repeats (GA)8C, (AG)8YT, (GA)8YC, (AG)8C, (GTT)6 and (GT)8YC give least amplification. UPGMA dendrogram constructed between these accessions depicted three major clusters. Based on genetic origin and diversity index viz. ICC-14051, ICC-13441, ICC-15518, ICC-12537, and ICC-17121 recommended to be selected as a parent in future breeding programmes for chickpea.