alexa Genetic Diversity Analysis of Date Palm (Phoenix dactylifera L.) Cultivars from Morocco Using SSR Markers
ISSN: 2376-0214

Journal of Biodiversity, Bioprospecting and Development
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Research Article

Genetic Diversity Analysis of Date Palm (Phoenix dactylifera L.) Cultivars from Morocco Using SSR Markers

Amy Bodian1*, Marion Nachtigall2, Lothar Frese2, Mohammed Aziz Elhoumaizi3, Amina Hasnaoui3, Khadidiatou Ndoye Ndir4 and Djibril Sané1
1Department of Plant Biology, Faculty of Sciences and Technology University Cheikh Anta Diop of Dakar, BP 5005 Senegal
2Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Erwin-Baur-Str. 27, 06484 Quedlinburg, Germany
3Department of Biology, Faculty of Sciences University Mohammed 1st Oujda, Morocco
4Department of Crop Sciences, UFR Agronomic Sciences and Rural Development University of Thiès, Senegal
*Corresponding Author : Amy BODIAN
1Department of Plant Biology
Faculty of Sciences and Technology University
Cheikh Anta Diop of Dakar
BP 5005 Senegal
Tel: +221 33 825 04 43
Fax: 221 33 824 63 18
E-mail: [email protected] ; [email protected]
Received August 26, 2014; Accepted September 27, 2014; Published September 29, 2014
Citation: Bodian A, Nachtigall M, Frese L, Aziz Elhoumaizi M, HasnaouiA, et al. (2014) Genetic Diversity Analysis of Date Palm (Phoenix Dactylifera L.) Cultivars from Morocco Using SSR Markers. J Biodivers Biopros Dev 1:126. doi:10.4172/2376-0214.1000126
Copyright: © 2014 Bodian A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

Abstract
In Morocco, date palm is the most important arboricultural crop and little is known about its germplasm. Thus, this work aimed at analyzing genetic diversity among 200 date palms sampled from three oases (Figuig, Tata and Zagora) of Morocco using microsatellite markers. Among these palms, 191 were females, belonging to 26 cultivars, and 9 were males. Eighteen primers were used for the analysis of their genetic diversity. Only 15 primers amplified successfully all the samples. The total number of alleles was 116 and the percentage of polymorphic loci was high and ranged between 60 and 100% with an average of 93.33%. The genetic similarity values ranged from 0.146 to 0.745. The molecular variance analysis showed 64% of variability among cultivars. The obtained dendrogram showed three groups and generally, a good structuring of cultivars. However, we noticed one case of homonymy among cultivars. In fact “Tadmant” cultivar of Figuig was different from “Tadmant” of Tata and Zagora. Males were clustered in two main subgroups.

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