Genetic Diversity and Complexity of Plasmodium Falciparum Infections in Lagos, Nigeria
- *Corresponding Author:
- Muyiwa K Oyebola
Parasitology and Bioinformatics
Faculty of Science University of Lagos, Nigeria
E-mail: [email protected]
Received date: November 27, 2013; Accepted date: August 28, 2014; Published date: September 01, 2014
Citation: Oyebola MK, Idowu TE, Olukosi AY, Agomo CO, Ajibaye OO, et al. (2014) Genetic Diversity and Complexity of Plasmodium Falciparum Infections in Lagos, Nigeria. J Bacteriol Parasitol 5:196. doi: 10.4172/2155-9597.1000196
Copyright: © 2014 Oyebola MK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Genetic diversity in Plasmodium falciparum populations is of major importance in the outcome of antimalarial drug trialsand vaccine design. Merozoite surface protein-1 (msp 1) and msp 2 are well known antigenic markers for distinguishing persistent and new infections with P. falciparum. Methods: Parasite DNA was extracted from one hundred blood samples collected from patients confirmed by microscopy to be P. falciparum-positive followed by PCR-genotyping for msp 1 (block2) and msp 2 (block 3) allelic families. Results: All the families of msp 1 locus (K1, MAD20 and R033) and msp 2 (FC27 and 3D7) were observed. K1 was the most predominant msp 1 allelic family (60/100) followed by MAD20 (50/100) while R033 had the least frequency (45/100). In the msp 2 locus, FC27 showed higher frequency (62/100) than 3D7 (55/100). The allelic families existed alone and/or in combination with other families. However, no R033/MAD20 combination was observed. Multiplicity of Infection (MOI) with msp 1 was higher in Ikorodu (1.50) than in Lekki (1.39) while MOI with msp 2 was lower in Ikorodu (1.14) than in Lekki (1.76). There was however no significant difference in the mean MOI between the two study areas (P=0.427). Conclusion: The observation of limited parasite diversity may signal the need for the use of less subjective genotyping tools in distinguishing recrudescence and reinfections with P. falciparum during drug trials.