alexa Genetic Diversity and Drug Resistance of 133 Mycobacterium tuberculosis Isolates from Jiangxi Province, China
ISSN: 2168-9547

Molecular Biology: Open Access
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Research Article

Genetic Diversity and Drug Resistance of 133 Mycobacterium tuberculosis Isolates from Jiangxi Province, China

Minghui Zhao1, Yang Liu2, Kaiming Liu1*, Kanglin Wan3, Zhiping Lv1, Shaohua Tu2, Yun Liao1 and Lin Ma4

1Jiangxi International Travel Healthcare Center, Jiangxi Entry-Exit Inspection and Quarantine Bureau, China

2Jiangxi Chest Hospital, China

3Chinese Center for Disease Control and Prevention, China

4The First Affiliated Hospital of Nanchang University, China

*Corresponding Author:
Kaiming Liu
Jiangxi International Travel Healthcare Center Jiangxi Entry
Exit Inspection and Quarantine Bureau, China
Tel: 0791-88328330
Fax: 0791-88326393
E-mail: [email protected]

Received date: February 18, 2016; Accepted date: February 23, 2016; Published date: March 01, 2016

Citation: Zhao M, Liu Y, Liu K, Wan K, Lv Z, et al. (2016) Genetic Diversity and Drug Resistance of 133 Mycobacterium tuberculosis Isolates from Jiangxi Province, China. Mol Biol 5:157. doi: 10.4172/2168-9547.1000157

Copyright: © 2016 Zhao M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

The genotypes of Mycobacterium tuberculosis (M. tuberculosis) have been found to differ in their resistance to different drugs. Although there is a high incidence of tuberculosis (TB) in Jiangxi, knowledge of the genotypes of M. tuberculosis in this province is limited in recent years. In this study, we investigated the relationship between genetic diversity and drug resistance in M. tuberculosis isolates collected from Jiangxi during January to October, 2014. A total of 133 M. tuberculosis isolates collected from the Jiangxi Chest Hospital were genotyped using both spacer oligonucleotide typing (spoligotyping) and 24-locus mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR). The resistance of these isolates to four first-line, and four second-line, anti-TB drugs, namely, isoniazid, rifampicin, ethambutol, streptomycin, capreomycin, amikacin, levofloxacin and protionamide, was then tested. The results indicate that the Beijing family was the most prevalent genotype (75.94%), followed by the T1 family (13.53%), the MANU2 family (1.50%), the T2 family (0.75%) and the Beijing-like genotype (0.75%). We also found nine new genotypes that did not match those in the Spoldb4.0 database. The 24-locus MIRU-VNTR method had a low clustering rate (10.53%) and a high HGDI (0.9723), and proved a high resolution method for genotyping M. tuberculosis isolates. More than half of the M. tuberculosis isolates collected in the present study were resistant to anti-TB drugs (56.39%, 75/133), and the majority of these were resistant to more than one drug (81.33%, 61/75). The Beijing family is the most prevalent TB strain in Jiangxi province, China. More than half the M. tuberculosis isolates collected were resistant to anti-TB drugs, and the majority was resistant to more than one drug. There was, however, no relationship between genetic diversity and drug resistance. Moreover, our results suggest that treatment history can lead to the development of drug resistance (P < 0.05), which supports the more moderate use of medication in the treatment of TB.

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