Genetic Diversity of Arabian Horse from Stud "Borike" (Bosnia and Herzegovina) Using Microsatellite MarkersDunja Rukavina1*, Danica Hasanbašić1, Adaleta Durmić-Pašić2, Belma Kalamujić2, Amir Zahirović3, Jasmin Ramić2 and Naris Pojskić2
- *Corresponding Author:
- Rukavina Dunja
Department for Biology, Veterinary Faculty, University of Sarajevo, Zmaja od Bosne 90, Bosnia and Herzegovia
E-mail: [email protected]
Received: 27/10/2015 Accepted: 18/11/2015 Published: 20/11/2015
The first evaluation of genetic diversity of Arabian horse from stud "Borike" (Bosnia and Herzegovina) was performed in this study. Genomic DNA was extracted from whole blood collected from 20 Arabian horses. A total of 17 microsatellite loci amplified by PCR. Average number of alleles per locus was 4.29, varying from 3 (HMS7, AHT5, HMS3, HMS1 and CA425) to 8 (HTG6). The observed heterozygosity ranged from 0.4 (HMS1) to 0.8 (ASB23, HTG7) with a mean of 0.6291 while expected heterozygosity ranged from 0.5088 (HMS1) to 0.7938 (HTG6) with a mean of 0.6529. The PIC values fluctuated from 0.4023 (HMS1) to 0.7643 (HTG6) with a mean of 0.5901. The inbreeding coefficient ranged from -0.0082 (CA425) to 0.4213 (HTG10) with a mean of 0.0621. Deviation from Hardy-Weinberg equilibrium (p<0,05) was found in five loci (HTG6, HTG10, HTG7, ASB17 and LEX3). HTG6 locus showed the highest, while the locus HMS1 showed the lowest genetic diversity. The results suggest that major loss of genetic diversity does not affect the population of Arabian horse sampled from stud “Borike” and indicate that Arabian horse from Bosnia and Herzegovina belongs to the modern Arabian horse populations.