alexa Genetic Expression Profile Analysis of the Temporal Inh
ISSN: 2329-8901

Journal of Probiotics & Health
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Research Article

Genetic Expression Profile Analysis of the Temporal Inhibition of Quercetin and Naringenin on Lactobacillus Rhamnosus GG

Linshu Liu1*, Jenni Firrman2, Gustavo Arango Argoty3, Peggy Tomasula1, Masuko Kobori4, Liqing Zhang3 and Weidong Xiao5*
1School of Medicine, Temple University, 3400 N Broad St, Philadelphia, PA 19140, USA
2Dairy and Functional Foods Research Unit, Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 600 E Mermaid Lane, Wyndmoor, PA 19038, USA
3Virginia Tech College of Engineering, Department of Computer Science, 1425 S Main St. Blacksburg, VA 24061, USA
4National Food Research Institute, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-8642, Japan
5*Department of Microbiology and Immunology, Temple University School of Medicine, 3400 North Broad Street, Philadelphia, USA
*Corresponding Author : Weidong Xiao
Department of Microbiology and Immunology
Temple University School of Medicine
3400 North Broad Street, Philadelphia, USA
Tel: 215-707-6392
E-mail: [email protected]
Received: January 29, 2015 Accepted: February 15, 2016 Published: February 22, 2016
Citation: Liu L, Firrman J, Argoty GA, Tomasula P, Kobori M, et al. (2016) Genetic Expression Profile Analysis of the Temporal Inhibition of Quercetin and Naringenin on Lactobacillus Rhamnosus GG. J Prob Health 4:139. doi:10.4172/2329-8901.1000139
Copyright: © 2016 Liu LS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

Plant polyphenols quercetin and naringenin are considered healthy dietary compounds; however, little is known of their effect on the probiotic Lactobacillus rhamnosus GG (LGG). In this study it was discovered that both quercetin and naringenin produced temporary inhibition of LGG growth, particularly at 8 hours post inoculation, with LGG eventually recovering from this suppression. The observed growth inhibition was regarded as a phenotypic response of LGG to the polyphenols; we hypothesized that the subsequent recovery was due to unknown, underlying genetic factors. The molecular response of LGG to quercetin and naringenin was determined through RNA analysis using the Helicos single molecule sequencing platform. The expression profiles of LGG grown in the presence of either quercetin or naringenin were divergent from each other, with only a few similarities, indicating that these polyphenols inhibit growth through separate mechanisms. LGG treated with quercetin demonstrated upregulation of genes associated with DNA repair and transcriptional regulation, and a decrease in expression of genes involved in metabolism and protein movement through the cell wall. LGG treated with naringenin resulted in an increase of genes associated with metabolism, and a decrease in genes involved in stress response. Results from this study demonstrate that there is a clear interaction between the polyphenols quercetin and naringenin and the probiotic LGG. The RNA expression analysis provides unique insight into the molecular response of LGG to quercetin and naringenin, revealing an identifiable pattern of gene expression.

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