Genetic Transformation of Cells using Physical MethodsRivera AL1*, Gómez-Lim M2, Fernández F2 and Loske AM1
- *Corresponding Author:
- Ana Leonor Rivera
UNAM, Queretaro, Mexico
E-mail: [email protected]
Received date: May 14, 2014; Accepted date:July 26, 2014; Published date: July 30, 2014
Citation: Rivera AL, Gomez-Lim M, Fernandez F, Loske AM (2014) Genetic Transformation of Cells using Physical Methods. J Genet Syndr Gene Ther 5:237. doi: 10.4172/2157-7412.1000237
Copyright: © 2014 Rivera AL, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The production of transgenic cells is a routinely process that allows to insert genes from plants, fungi, viruses, bacteria and even animals into cells. Genetic transformation requires penetration of the transgene through the cell wall. This process is facilitated by biological, chemical or physical methods. We present a short review of the state of the art of physical methods used for genetic transformation. A general panorama of the traditional physical genetic transformation methods like electroporation, biolistic, and agitation with glass beads, vacuum infiltration, silicon carbide whisker, laser microbeams, ultrasound, and the newly promising technique of shock wave-mediated genetic transformation of cells are described. These techniques have been applied to transform cells of bacteria, algae, plants, fungi, and animals. In human cells, genetic transformation is currently used for DNA vaccines, tissue engineering, and cancer therapy.