alexa Genomic Damage in Human Sperm Cells Exposed In Vitro to Environmental Pollutants | OMICS International | Abstract
ISSN: 2161-0525

Journal of Environmental & Analytical Toxicology
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Research Article

Genomic Damage in Human Sperm Cells Exposed In Vitro to Environmental Pollutants

Lucia Rocco1,2*, Carmela Peluso1, Fulvio Cesaroni3, Natascia Morra1, Daniela Cesaroni1 and Vincenzo Stingo1,2
1Department of Life Sciences, Second University of Naples, SUNFert Laboratory, Caserta, Italy
2IC-REACH, Interuniversitary Center Reach, University of Naples, Italy
3AORN “Sant’Anna e San Sebastiano”, Caserta, Italy
Corresponding Author : Lucia Rocco
SUNFert, Second University of Naples
Department of Life Sciences, Via Vivaldi 43
81100, Caserta, Italy
Tel: +39 0823274555
Fax: +39 0823274571
E-mail: [email protected]
Received September 21, 2011; Accepted January 10, 2012; Published January 12, 2012
Citation: Rocco L, Peluso C, Cesaroni F, Morra N, Cesaroni D, et al. (2012) Genomic Damage in Human Sperm Cells Exposed In Vitro to Environmental Pollutants. J Environment Analytic Toxicol 2:117. doi: 10.4172/2161-0525.1000117
Copyright: © 2012 Rocco L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Environmental pollution of waters caused by the release of chemical substances is becoming a threat for both man and the environment. The active pharmacological agents discharged into the waters, can interact with the cell DNA and induce genetic damage. The aim of the present work was to evaluate the potential genotoxic effects of nine pharmacological agents, Atorvastatin, Sildenafil citrate, Gemfibrozil, Ibuprofen, Atenolol, Ofloxacin, Carbamazepine, Bezafibrate, and Diclofenac present in the waste waters of some Italian treatment plants. Genotoxic effects of the nine pharmacological compounds were evaluated by using four tests, the Comet Assay, the Diffusion Assay, the TUNEL test, and RAPD-PCR technique. The sperm cells were exposed in vitro for three different times: 15, 30 and 45 min at the mean concentrations detected in the waste waters. All drugs tested induced a statistically significant reduction in the integrity of sperm DNA and high fragmentation values. The drugs found in the wastewater were then able to induce damage to sperm DNA, highlighted in a very early stage of cell suffering, even before they are capable of inducing other metabolic disorders.

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