alexa Genomic Damage in Human Sperm Cells Exposed In Vitro to Environmental Pollutants | OMICS International| Abstract
ISSN: 2161-0525

Journal of Environmental & Analytical Toxicology
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
  • Research Article   
  • J Environment Analytic Toxicol 2012, Vol 2(1): 117
  • DOI: 10.4172/2161-0525.1000117

Genomic Damage in Human Sperm Cells Exposed In Vitro to Environmental Pollutants

Lucia Rocco1,2*, Carmela Peluso1, Fulvio Cesaroni3, Natascia Morra1, Daniela Cesaroni1 and Vincenzo Stingo1,2
1Department of Life Sciences, Second University of Naples, SUNFert Laboratory, Caserta, Italy
2IC-REACH, Interuniversitary Center Reach, University of Naples, Italy
3AORN “Sant’Anna e San Sebastiano”, , Caserta, Italy
*Corresponding Author : Lucia Rocco, SUNFert, Second University of Naples, Department Of Life Sciences, Via Vivaldi 43, 81100, Caserta, Italy, Tel: +39 0823274555, Fax: +39 0823274571, Email: [email protected]

Received Date: Sep 21, 2011 / Accepted Date: Jan 10, 2012 / Published Date: Jan 12, 2012

Abstract

Environmental pollution of waters caused by the release of chemical substances is becoming a threat for both man and the environment. The active pharmacological agents discharged into the waters, can interact with the cell DNA and induce genetic damage. The aim of the present work was to evaluate the potential genotoxic effects of nine pharmacological agents, Atorvastatin, Sildenafil citrate, Gemfibrozil, Ibuprofen, Atenolol, Ofloxacin, Carbamazepine, Bezafibrate, and Diclofenac present in the waste waters of some Italian treatment plants. Genotoxic effects of the nine pharmacological compounds were evaluated by using four tests, the Comet Assay, the Diffusion Assay, the TUNEL test, and RAPD-PCR technique. The sperm cells were exposed in vitro for three different times: 15, 30 and 45 min at the mean concentrations detected in the waste waters. All drugs tested induced a statistically significant reduction in the integrity of sperm DNA and high fragmentation values. The drugs found in the wastewater were then able to induce damage to sperm DNA, highlighted in a very early stage of cell suffering, even before they are capable of inducing other metabolic disorders.

Keywords: DNA damage; Comet assay; Tunel test; RAPD-PCR; Male; Fertility; Environment

Citation: Rocco L, Peluso C, Cesaroni F, Morra N, Cesaroni D, et al. (2012)Genomic Damage in Human Sperm Cells Exposed In Vitro to EnvironmentalPollutants. J Environment Analytic Toxicol 2:117. Doi: 10.4172/2161-0525.1000117

Copyright: © 2012 Rocco L, et al. This is an open-access article distributed underthe terms of the Creative Commons Attribution License, which permits unrestricteduse, distribution, and reproduction in any medium, provided the original author andsource are credited.

Select your language of interest to view the total content in your interested language

Post Your Comment Citation
Share This Article
Recommended Conferences
Article Usage
  • Total views: 13303
  • [From(publication date): 1-2012 - Feb 21, 2020]
  • Breakdown by view type
  • HTML page views: 9430
  • PDF downloads: 3873
Top