alexa Genomic Instability in Embryonic Stem Cell: A Mechanism for Adaptation and Pluripotency Maintenance
ISSN: 2375-4508

Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biology
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Genomic Instability in Embryonic Stem Cell: A Mechanism for Adaptation and Pluripotency Maintenance

Clara I Esteban-Pérez1,2*, Harold H Moreno-Ortiz1,2, Carolina Lucena2, Nancy A Reichert1 and Dwayne A Wise1
1Department of Biological Sciences, Mississippi State University, Mississippi State, MS, 39762, USA
2Reproductive Biomedicine, Colombian Center of Fertility and Sterility, CECOLFES, Bogotá, Colombia, South America, USA
*Corresponding Author : Clara I. Esteban-Pérez
Colombian Center of Fertility and Sterility
CECOLFES, Bogotá, Colombia, South America
Tel: 571-7420505
Fax: 571- 7422235
Received December 18, 2014; Accepted February 26, 2015; Published March 05, 2015
Citation: Esteban-Pérez CI, Moreno-Ortiz HH, Lucena C, Reichert NA, Wise DA(2015) Genomic Instability in Embryonic Stem Cell: A Mechanism for Adaptation and Pluripotency Maintenance. J Fertil In Vitro IVF Worldw Reprod Med Genet Stem Cell Biol 3:142. doi:10.4172/2375-4508.1000142
Copyright: © 2015 Esteban-Pérez CI, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Embryonic stem (ES) cells have the ability to maintain pluripotency and self-renewal during in vitro maintenance, which is a key to their clinical applications. ES cell quality has been widely evaluated through determination of their specific genetic and epigenetic profiles. The hypothesis of this study is that genetic stability in repetitive sequences located near key genes involved in pluripotency, self-renewal, differentiation, chromatin assembly, and imprinting could be a signal for adaptation of the ES cell in vitro. Instability in specific repetitive sequences is present and increases during ES cell passages. ES cells displayed significant mean frequencies of instability in twelve markers out of 64 related to pluripotency (OCT4, D1S551), early differentiation (G60405, D18S63, and D1S468), chromatin assembly (D22S447, D6S2252, D10S529, and HISTB2), and imprinting (GRB10-promoter, D2S144, and IGF2- promoter). Interestingly, instability was different between H1 and H7 ES cell lines. In summary, these results suggest that instability in tandem repeat sequences located near early embryonic developmental genes is associated with failure of ES cell pluripotency and self-renewal maintenance over consecutive culture passages. These results suggest that instability determination is a potential indicator of gene deregulation and epigenetic modification that involves chromatin modification and imprint establishment during ES cell culture. Finally, instability in specific genes could be a signal that contributes to adaptation of ES cells to in vitro culture or could be the switch that initiates early cell specialization in vitro.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

1-702-714-7001Extn: 9037

Business & Management Journals


1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version