alexa Genotoxicity and Histopathological Studies on the Liver
ISSN: 2157-7099

Journal of Cytology & Histology
Open Access

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Research Article

Genotoxicity and Histopathological Studies on the Liver, Kidney and Lymphocytes of Male Rats Fed on Diet Containing Waste Fat Released from Chicken During Grilling Process

Hassan GM1* and Mazher KHM2

1Genetics Dept., Fac. Agric., Fayoum Univ., Egypt

2Cytol. Histol. Dept., Fac.Vet. Med., Beni Suif Univ., Egypt

*Corresponding Author:
Hassan GM
Genetics Dept., Fac. Agric.
Fayoum Univ., Egypt
Tel: +2-084-6380655
Fax: +2-084-6334964
E-mail: [email protected], [email protected]

Received date: December 05, 2010; Accepted date: February 09, 2011; Published date: February 11, 2011

Citation: Hassan GM, Mazher KHM (2011) Genotoxicity and Histopathological Studies on the Liver, Kidney and Lymphocytes of Male Rats Fed on Diet Containing Waste Fat Released from Chicken During Grilling Process. J Cytol Histol 2:111. doi:10.4172/2157-7099.1000111

Copyright: © 2011 Hassan GM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Fat released from chicken during grilling process, as waste, is used by some low income people in Egypt in cooking as dietary fat because it is a cheap fat source compared to other fats. The present study aimed to elucidate the genotoxic and histopatholgic effects of grilled chicken fat on the liver, kidney and lymphocytes of male albino rats. Fifteen two months old male rats were divided to three groups, the first group was fed on control diet, the second and third groups were fed diets containing wasted fat at substitution levels of 50 and 100% from the fat of diet, respectively. The animals were fed ad-libtium for eight weeks. The single cell gel electrophoresis (Comet) assay is a simple and effective method for detecting DNA damage in control and treated rat cells, and results showed significant increase in tailed nuclei (DNA damage), tail moment , % DNA in the tail and tail length in liver , kidney and lymphocytes of treated groups compared to the control.In addition, both liver and kidney of treated rats showed a marked degenerative changes congestion of blood vessels in addition to loss of protein, polysaccharides and DNA content.

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