alexa Genotypic and phenotypic diversity of Bacillus spp. isolated from Freshwater Ecosystems | OMICS International | Abstract
ISSN: 2155-9546

Journal of Aquaculture Research & Development
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Genotypic and phenotypic diversity of Bacillus spp. isolated from Freshwater Ecosystems

Mohanty S, Choudhury P. K, Dash A, Samanta M and Maiti N. K*

A-Microbiology section, Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Orissa, India, 751002

*Corresponding Author:
Dr. N. K. Maiti
Principal Scientist, Microbiology section
Division of Fish Health Management
Central Institute of Freshwater Aquaculture
Kausalyaganga, Bhubaneswar, Orissa, India, 751002
Tel: 91-674- 2465446
Fax: 91-674-2465407
E-mail: [email protected]

Received Date: December 14, 2010; Accepted Date: January 20, 2011; Published Date: May 11, 2011

Citation: Mohanty S, Choudhury PK, Dash A, Samanta M, Maiti NK (2011) Genotypic and phenotypic diversity of Bacillus spp. isolated from Freshwater Ecosystems. J Aquac Res Development 2:112. doi:10.4172/2155-9546.1000112

Copyright: © 2011 Mohanty S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The efficacy of the restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction (PCR) amplified rrs (16S ribosomal ribonucleic acid [rRNA] gene) for the identification of Bacillus spp. isolated from freshwater pond ecosystems was evaluated. Results showed that no restriction site differences occur between rrs operons within the bacteria belonging to the same species and as a result members of the same species yield very similar RFLP patterns at 100% similarity level. Cluster analysis of PCR-RFLP patterns grouped all the isolates into three clusters; each cluster represented a single species except one for which no placement was obtained. Phenotypic characteristics of the isolates were found to be well concordance with the results of PCR-RFLP. Our findings suggested that PCR- RFLP is suitable tool for discrimination and identification of endoglucanase positive Bacillus spp. isolated from freshwater ecosystems.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7