Ginkgo Biloba Extract Protects Substantia Nigral Neurons from Apoptosis in PD Rat Model
|Cao Fei*, Luo Fang, Zhang Lei, Su Ying, Chen Li, Hu Bo and Tong E-tang|
|Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China|
|Corresponding Author :||Cao Fei
Department of Neurology
Union Hospital, Tongji Medical College
Huazhong University of Science and Technology
E-mail: [email protected]
|Received April 11, 2013; Accepted May 27, 2013; Published May 29, 2013|
|Citation: Fei C, Fang L, Lei Z, Ying S, Li C, et al. (2013) Ginkgo Biloba Extract Protects Substantia Nigral Neurons from Apoptosis in PD Rat Model. J Neurol Disord 1:118. doi: 10.4172/2329-6895.1000118|
|Copyright: © 2013 Fei C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Purpose: To investigate the apoptotic mechanism during the pathological process of Parkinson disease (PD) and explore the availability of Ginkgo biloba extract (EGb) to cure PD.
Methods: Followed 6-OHDA injection stereotaxically to right side of substantia nigra pars compacta(SNc), at the date of 1, 7,14 and 21, we used biochemical, immunohistochemical, TUNEL and electron microscopic techniques to evaluate the level of apoptosis (TUNEL), monitor the degree of oxidative reaction (MDA.SOD), and to observe the change of ultrastructure of substantia nigra in the experimental PD models of rats (PD group), the EGb treatment group (EGb group) and the control group. Results: Contrast to the Control group, the number of apoptotic neurons and free radical level increased in the PD group and the EGb group, but the PD group surpassed the EGb group (p<0.05).In the PD group, the ultrastructure of substantia nigra exhibited the morphological characteristics of apoptosis from 1d to 21d.
Conclusions: Free radical insult contributes to the pathological process of PD in rats. EGb may ameliorate the injury of substantia nigra through expiring free radicals during the pathological process of PD in rats.