alexa Gluconic Acid Production from Golden Syrup by Aspergillus niger Strain using Semiautomatic Stirred-Tank Fermenter | OMICS International
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
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Research Article

Gluconic Acid Production from Golden Syrup by Aspergillus niger Strain using Semiautomatic Stirred-Tank Fermenter

Nilesh K Purane1,2*, Shital K Sharma1, Prajakta D Salunkhe3, Dinesh S Labade4 and Monali M Tondlikar5

1Bioprocessing & Herbal Division, Mahatma Gandhi Institute for Rural Industrialization, Wardha-442 001, India

2Department of Biotechnology, Kala Biotech, Pune, India

3Department of Biotechnology, Lokamangal Biotechnology College, Solapur-413 222, India

4Department of Biotechnology, Vidya Pratisthan’s School of Biotechnology, Baramati-413 133, India

5Department of Biotechnology, Kala Biotech, Pune-410 507, India

*Corresponding Author:
Nilesh K Purane
Department of Biotechnology
Kala Biotech
Pune-410 507 India
E-mail: [email protected]

Received Date: May 06, 2012; Accepted Date: August 13, 2012; Published Date: August 16, 2012

Citation: Purane NK, Sharma SK, Salunkhe PD, Labade DS, Tondlikar MM (2012) Gluconic Acid Production from Golden Syrup by Aspergillus niger Strain Using Semiautomatic Stirred-Tank Fermenter. J Microb Biochem Technol 4:092-095. doi: 10.4172/1948-5948.1000077

Copyright: © 2012 Purane NK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The gluconic acid batch fermentation was conducted using mutant Aspergillus niger NCIM 530 strain under submerged condition in 50 L semiautomatic stirred-tank fermenter. Certain cost-effective source as golden syrup was effectively utilized instead of glucose for successful industrial fermentation process. The significant level of gluconic acid (85.2 gL-1) production was observed with maximum 86.97% glucose conversion over 44 hours. This process provides great advantages over traditional submerged fermentation strategies and substrates, as showed by effective production of gluconic acid by utilizing novel substrate as a golden syrup. To reduce analysis time with better accuracy, an effort has been made to use a method for evaluation of parameters like conversion of substrate and production of gluconic acid during the fermentation by using High Performance Thin Layer Chromatography (HPTLC).

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